Biomaterial Database

Regulatory effect of arachidonic acid on the calcium transport system in rat liver nuclei. 1993

K Oishi, and M Yamaguchi

Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.

The effect of arachidonic acid (AA) on Ca2+ transport in rat liver nuclei was investigated. Ca2+ uptake and release were determined with a Ca2+ electrode. Ca2+ uptake increased dependent on ATP (0.5-2.0 mM), while uptake was negligible in the presence of 2.0 mM ADP or AMP. AA (10-100 microM) caused a marked inhibition of Ca2+ uptake following the addition of 2.0 mM ATP. Also, Ca2+, which accumulated in the nuclei during 6 min after ATP addition, was clearly released by the addition of AA (10-100 microM). The alterations were concentration dependent. The nuclear Ca2+ uptake and release were not altered significantly by the presence of prostaglandin E2 (10 and 20 microM), prostaglandin H2 (1 and 4 microM), thromboxane B2 (1 and 4 microM), leukotriene A4 (1 and 4 microM), Ins(1,4,5)P3 (1 and 10 microM) or dibutyryl cAMP (10 and 50 microM). Only, 5-hydroxy-eicosatetraenoic acid (5-HETE) at 4 microM caused a significant inhibition of nuclear Ca2+ uptake and an appreciable increase in Ca2+ release; the 1 microM concentration had no effect. These results indicate that AA, one of the prostanoids, has a unique effect on Ca2+ uptake and release in rat liver nuclei. The finding suggests that AA has a regulatory effect on the Ca2+ transport system in liver nuclei.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D011453	Prostaglandins	A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.	Prostaglandin,Prostanoid,Prostanoids
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D004229	Dithiothreitol	A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols.	Cleland Reagent,Cleland's Reagent,Sputolysin,Clelands Reagent,Reagent, Cleland,Reagent, Cleland's
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004347	Drug Interactions	The action of a drug that may affect the activity, metabolism, or toxicity of another drug.	Drug Interaction,Interaction, Drug,Interactions, Drug
D006893	Hydroxyeicosatetraenoic Acids	Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.	HETE,Acids, Hydroxyeicosatetraenoic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia