Biomaterial Database

Crystallization of phosphatidylinositol-specific phospholipase C from Bacillus cereus. 1993

T L Bullock, and M Ryan, and S L Kim, and S J Remington, and O H Griffith

Institute of Molecular Biology, University of Oregon, Eugene 97403.

Phosphatidylinositol-specific phospholipase C (PI-PLC) cleaves phosphoinositides into two parts, lipid-soluble diacylglycerol and the water-soluble phosphorylated inositol. Two crystal forms of Bacillus cereus PI-PLC have been obtained by the vapor diffusion technique. Hexagonal crystals were grown from solutions containing polyethylene glycol (PEG; 4,000 to 8,000 D). The space group of these hexagonal crystals is P6(1)22 (or the enantiomorphic space group P6(5)22), with cell constants a = b = 133 A, and c = 231 A. The crystals diffract to 2.8 A. The second crystalline form was grown from a two-phase PEG (600 D)-sodium citrate solution. The phase diagram and PI-PLC distribution between phases has been determined. The enzyme crystallizes from the PEG-rich phase. The crystals are orthorhombic with space group P2(1)2(1)2(1) (a = 45 A, b = 46 A, c = 160 A), and contain one PI-PLC monomer per asymmetric unit. The orthorhombic crystals diffract to 2.5 A. Both the hexagonal and orthorhombic forms are suitable for crystallographic studies.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010727	Phosphoric Diester Hydrolases	A class of enzymes that catalyze the hydrolysis of one of the two ester bonds in a phosphodiester compound. EC 3.1.4.	Phosphodiesterase,Phosphodiesterases,Hydrolases, Phosphoric Diester
D011092	Polyethylene Glycols	Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.	Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D002951	Citrates	Derivatives of CITRIC ACID.
D003460	Crystallization	The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D001409	Bacillus cereus	A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.
D001703	Biophysics	The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.	Mechanobiology
D014961	X-Ray Diffraction	The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions
D043265	Phosphatidylinositol Diacylglycerol-Lyase	A phosphorus-oxygen lyase found primarily in BACTERIA. The enzyme catalyzes the cleavage of a phosphoester linkage in 1-phosphatidyl-1D-myo-inositol to form 1D-myo-inositol 1,2-cyclic phosphate and diacylglycerol. The enzyme was formerly classified as a phosphoric diester hydrolase (EC 3.1.4.10) and is often referred to as a TYPE C PHOSPHOLIPASES. However it is now known that a cyclic phosphate is the final product of this enzyme and that water does not enter into the reaction.	1-Phosphatidylinositol Phosphodiesterase,Monophosphatidylinositol Phosphodiesterase,1 Phosphatidylinositol Phosphodiesterase,Diacylglycerol-Lyase, Phosphatidylinositol,Phosphatidylinositol Diacylglycerol Lyase,Phosphodiesterase, 1-Phosphatidylinositol,Phosphodiesterase, Monophosphatidylinositol
D054801	Phosphoinositide Phospholipase C	A type C phospholipase with specificity towards PHOSPHATIDYLINOSITOLS that contain INOSITOL 1,4,5-TRISPHOSPHATE. Many of the enzymes listed under this classification are involved in intracellular signaling.	1-Phosphatidyl-D-myo-Inositol-4,5-bisphosphate Inositoltrisphosphohydrolase,1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase,PIP2-PLC,PIP2-Phosphoinositidase C,PPI Phosphodiesterase,Phosphatidylinositide-4,5-Bisphosphate Phospholipase C,Phosphatidylinositol-4,5-Biphosphate-Phosphodiesterase,Phosphatidylinositol-Specific Phospholipase C,Phosphoinositide Selective Phospholipase C,Phosphoinositol Specific Phospholipase C,Polyphosphoinositide Phosphodiesterase,Polyphosphoinositide Phospholipase C,PtdIns(4,5)P2 Phosphodiesterase,Tri-phosphoinositide Phosphodiesterase,Triphosphoinositide Phosphodiesterase,1 Phosphatidyl D myo Inositol 4,5 bisphosphate Inositoltrisphosphohydrolase,1 Phosphatidylinositol 4,5 Bisphosphate Phosphodiesterase,Inositoltrisphosphohydrolase, 1-Phosphatidyl-D-myo-Inositol-4,5-bisphosphate,PIP2 Phosphoinositidase C,Phosphatidylinositide 4,5 Bisphosphate Phospholipase C,Phosphatidylinositol 4,5 Biphosphate Phosphodiesterase,Phosphatidylinositol Specific Phospholipase C,Phosphodiesterase, 1-Phosphatidylinositol-4,5-Bisphosphate,Phosphodiesterase, PPI,Phosphodiesterase, Polyphosphoinositide,Phosphodiesterase, Triphosphoinositide,Phospholipase C, Phosphatidylinositide-4,5-Bisphosphate,Phospholipase C, Phosphatidylinositol-Specific,Phospholipase C, Polyphosphoinositide,Tri phosphoinositide Phosphodiesterase