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The aim of the present study was to separate and characterise products formed by oxidation of uric acid by hydroxyl radicals with a view to probing for these products in vivo in clinical contexts. Aerated solutions of 200 microM uric acid, or its oxidation products, allantoin or parabanic acid, were exposed to gamma radiolysis, (52.0 Gy/min), as a source of HO. radicals, at pH 3.4 and 7.4. Aliquots were taken every 5 minutes for 20 minutes and oxidation products were separated by HPLC and analysed with a diode array detector. Identities of oxidation products were confirmed on the basis of similarity of retention times and absorbance spectra and peak purity parameters of known standards. Hydroperoxides were measured by tri-iodide formation in the 20 minute sample. Exposure of uric acid to such HO.fluxes produced a net loss of the parent compound with formation of a complex mixture of products with allantoin and parabanic acid being the predominant products at pH 3.4. The rate of uric acid degradation at physiological pH was slower and the distribution of oxidation products was different. A small but significant amount of uric acid hydroperoxide was detected at both pHs. A mechanism for uric acid oxidation under these conditions is presented.
M Hicks, and
L S Wong, and
R O Day
January 1990,
Chemico-biological interactions,
M Hicks, and
L S Wong, and
R O Day
January 1986,
Nucleic acids symposium series,
M Hicks, and
L S Wong, and
R O Day
February 1989,
FEBS letters,
M Hicks, and
L S Wong, and
R O Day
October 2013,
Chemosphere,
M Hicks, and
L S Wong, and
R O Day
October 1997,
Plant physiology,
M Hicks, and
L S Wong, and
R O Day
April 2010,
Geochemical transactions,
M Hicks, and
L S Wong, and
R O Day
June 2022,
Water research,
M Hicks, and
L S Wong, and
R O Day
March 2018,
Environmental science & technology,
M Hicks, and
L S Wong, and
R O Day
November 2018,
Environmental science and pollution research international,
M Hicks, and
L S Wong, and
R O Day
July 1965,
Biochemistry,
