Biomaterial Database

Interaction of crocidolite asbestos with hamster respiratory mucosa in organ culture. 1977

B T Mossman, and J B Kessler, and B W Ley, and J E Craighead

Organ cultures of hamster trachea were used to study the effects of crocidolite asbestos on the respiratroy epithelium and the uptake of asbestos by cells of the mucosa. International Union Against Cancer (U.I.C.C.) crocidolite was suspended in medium over a range of concentrations and precipitated on the epithelial surface for 1 hour. At intervals during the ensuing 4 weeks, morphologic changes were documented by light, scanning electron, and transmission electron microscopy. Cytotoxic alterations in differentiated mucosal cells appeared to relate to the amount of crocidolite added to the cultures. Necrosis and desquamation of surface cells were accompanied by basal cell hyperplasia. These proliferating cells phagocytosed the dust and incorporated it into lysosomes. Crocidolite was also found interposed between cells of the hyperplastic basal cell layer. Transport of asbestos particles to the submucosa and uptake by mesenchymal cells was apparent after 1 week.

UI	MeSH Term	Description	Entries
D009092	Mucous Membrane	An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa.	Lamina Propria,Mucosa,Mucosal Tissue,Muscularis Mucosae,Mucous Membranes,Membrane, Mucous,Membranes, Mucous,Mucosae, Muscularis,Mucosal Tissues,Propria, Lamina,Tissue, Mucosal,Tissues, Mucosal
D009924	Organ Culture Techniques	A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)	Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004848	Epithelium	The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body.	Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D001194	Asbestos	Asbestos. Fibrous incombustible mineral composed of magnesium and calcium silicates with or without other elements. It is relatively inert chemically and used in thermal insulation and fireproofing. Inhalation of dust causes asbestosis and later lung and gastrointestinal neoplasms.
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014132	Trachea	The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.	Tracheas