Biomaterial Database

Increase in the adenine nucleotide translocase protein contributes to the perinatal maturation of respiration in rat liver mitochondria. 1993

P Schönfeld, and S Fritz, and W Halangk, and R Bohnensack

Institut für Biochemie, Medizinischen Akademie Magdeburg, Germany.

An assay based on the high-affinity binding of tritium-labelled atractyloside to the adenine nucleotide translocase (ANT) was developed for estimation of its content in samples of mitochondria, cells and tissue homogenate. The assay was used to study the developmental change of the ANT protein concentration in perinatal rat liver. Within the last 3 days before birth the content of the ANT protein per mg tissue protein increased from 29 to 45% of the maximum value found 2 days after birth. A similar developmental change of the ANT protein was found in isolated mitochondria, demonstrating that the perinatal increase in the ANT protein content was due mainly to a mitochondrial differentiation process and not the result of an increase in the number of mitochondria per cell. A close proportionality between the ANT protein and the ADP-stimulated respiration of liver homogenate was found in the perinatal period from 3 days before to 2 days after birth. This finding suggests that the developmental change in the ANT protein content plays an important role in the onset of oxidative phosphorylation after birth.

UI	MeSH Term	Description	Entries
D008930	Mitochondria, Liver	Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)	Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D010085	Oxidative Phosphorylation	Electron transfer through the cytochrome system liberating free energy which is transformed into high-energy phosphate bonds.	Phosphorylation, Oxidative,Oxidative Phosphorylations,Phosphorylations, Oxidative
D010500	Perinatology	The branch of medicine dealing with the FETUS and NEWBORN during the perinatal period. The perinatal period begins with the twenty-eighth week of gestation and ends twenty-eight days after birth.	Fetal Medicine,Maternal-Fetal Medicine,Materno-Fetal Medicine,Fetal Medicines,Maternal Fetal Medicine,Maternal-Fetal Medicines,Materno Fetal Medicine,Materno-Fetal Medicines,Medicine, Fetal,Medicine, Maternal-Fetal,Medicine, Materno-Fetal
D005333	Fetus	The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.	Fetal Structures,Fetal Tissue,Fetuses,Mummified Fetus,Retained Fetus,Fetal Structure,Fetal Tissues,Fetus, Mummified,Fetus, Retained,Structure, Fetal,Structures, Fetal,Tissue, Fetal,Tissues, Fetal
D000226	Mitochondrial ADP, ATP Translocases	A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.	ADP,ATP Carrier,ADP,ATP Translocator Protein,Adenine Nucleotide Translocase,ADP Translocase,ATP Translocase,ATP,ADP-Carrier,ATP-ADP Translocase,Adenine Nucleotide Carrier (Mitochondrial),Mitochondrial ADP-ATP Carriers,ADP-ATP Carriers, Mitochondrial,Mitochondrial ADP ATP Carriers
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000831	Animals, Newborn	Refers to animals in the period of time just after birth.	Animals, Neonatal,Animal, Neonatal,Animal, Newborn,Neonatal Animal,Neonatal Animals,Newborn Animal,Newborn Animals
D001278	Atractyloside	A glycoside of a kaurene type diterpene that is found in some plants including Atractylis gummifera (ATRACTYLIS); COFFEE; XANTHIUM, and CALLILEPIS. Toxicity is due to inhibition of ADENINE NUCLEOTIDE TRANSLOCASE.	Atractylic Acid,Atractylate,Acid, Atractylic
D014316	Tritium	The radioactive isotope of hydrogen also known as hydrogen-3. It contains two NEUTRONS and one PROTON in its nucleus and decays to produce low energy BETA PARTICLES.	Hydrogen-3,Hydrogen 3
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats