Biomaterial Database

Purification and characterization of novel lectins from Great Northern bean, Phaseolus vulgaris L. 1993

K Kamemura, and Y Furuichi, and H Umekawa, and T Takahashi

Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.

Two lectins, GNL-1 and 2, were isolated from extracts of Great Northern bean powder through fractionation with ammonium sulfate, ion-exchange chromatographies on CM- and DEAE-celluloses, and gel filtration chromatography on Sephacryl S-200 HR. These lectins were shown to be homogenous by gel electrophoresis, gel filtration, and isoelectric focusing. The lectins (GNL-1 and 2) have molecular masses of 175 and 145 kDa on gel filtration, respectively. They yield three bands having the respective same molecular masses on SDS-PAGE (GNL-1; alpha-subunit of 34.5 kDa, beta of 37.0, and gamma of 39.0: GNL-2; alpha' of 34.5 kDa, beta' of 37.0, and gamma' of 39.0). Two lectins are shown to be glycoproteins and the carbohydrate contents of GNL-1 and 2 are 5.1 and 4.5%, respectively. The isoelectric points are 5.5 and 5.1 and the extinction coefficients (A 1cm 1%) at 280 nm are 11.37 and 11.45, respectively. These lectins are nonspecific in agglutination for rabbit and any types of human erythrocytes. Inhibition study shows no specificity against mono and disaccharides. On the other hand, binding assay of horseradish peroxidase-glycoproteins to the bands electroblotted onto PVDF membrane reveals that all of the subunits can bind to sugar moieties in fetuin, asialofetuin, and porcine thyroglobulin specifically. Moreover, assay of mitogenic activity shows that GNL-1 is a strong mitogen, but GNL-2 is lack of the activity.

UI	MeSH Term	Description	Entries
D007887	Fabaceae	The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.	Afzelia,Amorpha,Andira,Baptisia,Callerya,Ceratonia,Clathrotropis,Colophospermum,Copaifera,Delonix,Euchresta,Guibourtia,Legumes,Machaerium,Pithecolobium,Stryphnodendron,Leguminosae,Pea Family,Pithecellobium,Tachigalia,Families, Pea,Family, Pea,Legume,Pea Families
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010946	Plants, Medicinal	Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.	Herbs, Medicinal,Medicinal Herbs,Healing Plants,Medicinal Plants,Pharmaceutical Plants,Healing Plant,Herb, Medicinal,Medicinal Herb,Medicinal Plant,Pharmaceutical Plant,Plant, Healing,Plant, Medicinal,Plant, Pharmaceutical,Plants, Healing,Plants, Pharmaceutical
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D037102	Lectins	Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.	Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal
D037121	Plant Lectins	Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.	Lectins, Plant,Phytagglutinin,Plant Agglutinin,Plant Lectin,Agglutinins, Plant,Phytagglutinins,Plant Agglutinins,Agglutinin, Plant,Lectin, Plant