Biomaterial Database

Molecular cloning and characterization of PstI fragments of bovine herpesvirus-1 DNA. 1993

P K Gupta, and A Rai

National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar.

The DNA from an Indian isolate of BHV-1 was cleaved with the restriction enzyme PstI and cloned into pUC9 plasmid. Two recombinant plasmids were then analyzed using restriction endonucleases PstI, EcoRI, BamHI, SmaI, SalI, HindIII, BglI, HaeIII, XhoI, RsaI and StuI. On the basis of digested fragments of recombinant plasmids, two plasmids could be mapped for restriction enzymes SalI, StuI, SmaI and XhoI using single and double digestion.

UI	MeSH Term	Description	Entries
D007242	Herpesvirus 1, Bovine	A species of VARICELLOVIRUS that causes INFECTIOUS BOVINE RHINOTRACHEITIS and other associated syndromes in CATTLE.	IBR-IPV Virus,Infectious Bovine Rhinotracheitis Virus,Infectious Pustular Vulvovaginitis Virus,Bovine Herpesvirus 1,Bovine Rhinotracheitis Virus, Infectious,Herpesvirus 1 (alpha), Bovine,Pustular Vulvovaginitis Virus, Infectious,IBR IPV Virus,IBR-IPV Viruses,Virus, IBR-IPV,Viruses, IBR-IPV
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004274	DNA, Recombinant	Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.	Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D005822	Genetic Vectors	DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.	Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia