Decreased polymorphonuclear leukocyte exudation in critically ill anergic patients associated with increased adhesion receptor expression. 1993

J M Tellado, and G C McGowen, and N V Christou
Department of Surgery, McGill University, Montreal, PQ, Canada.

OBJECTIVE To determine the mechanism for the reduced polymorphonuclear leukocyte exudation in critically ill anergic patients. METHODS Prospective consecutive patient study. METHODS Tertiary care surgical intensive care unit. METHODS Eighteen patients with intra-abdominal injections were studied. METHODS Critically ill patients were stratified based on their delayed type hypersensitivity response to ubiquitous antigens. Polymorphonuclear leukocytes were isolated from blood and from exudate blister type skin windows. Adhesion and chemotactic surface receptors were measured, as was cytokine content and chemoattraction capacity of skin window fluid for control neutrophils. RESULTS Circulating neutrophils from anergic patients have increased CR3 adherence receptors compared with those neutrophils from reactive patients. f-met-leu-phe receptors are equal in number and C5a receptors are either significantly reduced in number or occupied with ligand. This same receptor pattern is maintained after neutrophil exudation in both patient groups. Serum and skin window fluid from anergic patients attracted less neutrophils in vitro and in vivo. CONCLUSIONS These data suggest two possible mechanisms for the reduced neutrophil delivery of critically ill anergic patients: a receptor-mediated increased adherence to vascular endothelium preventing diapedesis; reduced chemo-attraction potential of serum, and possibly, exudate fluid.

UI MeSH Term Description Entries
D006968 Hypersensitivity, Delayed An increased reactivity to specific antigens mediated not by antibodies but by sensitized T CELLS. Hypersensitivity, Tuberculin-Type,Hypersensitivity, Type IV,Tuberculin-Type Hypersensitivity,Type IV Hypersensitivity,Delayed Hypersensitivity,Delayed Hypersensitivities,Hypersensitivity, Tuberculin Type,Tuberculin Type Hypersensitivity,Tuberculin-Type Hypersensitivities,Type IV Hypersensitivities
D007108 Immune Tolerance The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc. Immunosuppression (Physiology),Immunosuppressions (Physiology),Tolerance, Immune
D008297 Male Males
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D002634 Chemotaxis, Leukocyte The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction. Leukotaxis,Leukocyte Chemotaxis
D005122 Exudates and Transudates Exudates are fluids, CELLS, or other cellular substances that are slowly discharged from BLOOD VESSELS usually from inflamed tissues. Transudates are fluids that pass through a membrane or squeeze through tissue or into the EXTRACELLULAR SPACE of TISSUES. Transudates are thin and watery and contain few cells or PROTEINS. Transudates,Exudates,Transudates and Exudates,Exudate,Transudate
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults

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