BIOMAT Database Logo BIOMAT Database Logo

Nonenzymatic advanced glycation in the lens membranes. 1993

J N Liang
Department of Surgery Ophthalmology, Brigham and Women's Hospital, Boston, MA 02115.

Nonenzymatic advanced glycation was induced in bovine lens membranes. The resulting modified membranes display some changes which were also observed in the advanced glycated crystallins: an increased absorption in the near-UV region, appearance of a strong blue fluorescence (Ex/Em = 370/450 nm), and a subtle change in the near-UV circular dichroism (CD). These changes were also observed in diabetic human lens membranes, but with a less extent in nondiabetic samples. The results indicate that advanced glycation is important in diabetes mellitus, not only to crystallins but also to membrane proteins, both of which generate yellow pigment chromophores that result in lens coloration.

UI MeSH Term Description Entries
D007908 Lens, Crystalline A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION. Eye Lens,Lens, Eye,Crystalline Lens
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006031 Glycosylation The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION). Protein Glycosylation,Glycosylation, Protein
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012266 Ribose A pentose active in biological systems usually in its D-form. D-Ribose,D Ribose
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry

Related Publications

J N Liang
Nonenzymatic glycosylation (glycation) of lens crystallins in diabetes and aging.
January 1989, Progress in clinical and biological research,
J N Liang
Nonenzymatic glycation of human lens crystallin. Effect of aging and diabetes mellitus.
November 1984, The Journal of clinical investigation,
J N Liang
Glycation of MP26 and MP22 in bovine lens membranes.
June 1992, Biochemical and biophysical research communications,
J N Liang
In vitro nonenzymatic glycation of DNA nucleobases: an evaluation of advanced glycation end products under alkaline pH.
November 2006, Analytical and bioanalytical chemistry,
J N Liang
Differences in nonenzymatic glycation of histones.
January 1994, Acta biochimica Polonica,
J N Liang
Advanced glycation end products in human diabetic lens capsules.
September 2021, Experimental eye research,
J N Liang
Nonenzymatic glycation alters protein structure and stability. A study of two eye lens crystallins.
August 1993, The Journal of biological chemistry,
J N Liang
Hesperetin treatment attenuates glycation of lens proteins and advanced‑glycation end products generation.
May 2023, Molecular medicine reports,
J N Liang
Nonenzymatic glycation of plasma proteins in smokers.
January 2006, Indian journal of physiology and pharmacology,
J N Liang
Role of polyol pathway in nonenzymatic glycation.
January 1996, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
Copied contents to your clipboard!