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We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of NotI site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G + C-rich templates were optimized to establish these NotI sites as sequence-tagged sites.
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
November 1993,
Genomics,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
January 1994,
DNA research : an international journal for rapid publication of reports on genes and genomes,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
May 1991,
Genomics,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
February 1993,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
May 1993,
Human molecular genetics,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
October 1992,
Genomics,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
January 1992,
Mammalian genome : official journal of the International Mammalian Genome Society,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
December 1992,
Genomics,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
August 1993,
Human molecular genetics,
M Hattori, and
A Toyoda, and
H Ichikawa, and
T Ito, and
H Ohgusu, and
N Oishi, and
T Kano, and
S Kuhara, and
M Ohki, and
Y Sakaki
August 1991,
Genomics,
