Six normal males underwent both penile vibratory stimulation and masturbation in order to compare the quantity and quality of semen produced by each method. There was no significant difference in the quantity and quality of the ejaculates produced by vibratory stimulation of the penis and masturbation. In addition, biochemical analysis of the seminal fluid collected by both procedures revealed similar values between all specimens for nine organic constituents, seven inorganic constituents and seven metabolic enzymes. None of the subjects demonstrated retrograde flow of semen. These findings indicate that vibratory stimulation is a 'physiological' means of inducing ejaculation, and can produce semen of normal quality.