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Field flow fractionation (FFF) is a new methodology described as being well-suited for the separation and characterization of biopolymers and particles. On theoretical grounds, cells may be separated with FFF if they differ in size, density or deformability. In the present study, we first tried to determine optimal separation conditions for red blood cells; thereafter we used FFF to examine red cell changes during a phenylhydrazine-induced hemolytic anemia. It has been shown that in less than 30 minutes, FFF is able to separate normal red blood cells from Heinz body-rich cells or reticulocytes that differ in size or density. The successive steps of hemolysis and regeneration appear clearly on the fractograms. Advantages and drawbacks of the method are discussed.
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
July 1991,
Journal of chromatography,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
November 1992,
Journal of chromatography,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
July 1993,
Analytical biochemistry,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
December 1995,
Journal of chromatography. B, Biomedical applications,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
September 2011,
Biomicrofluidics,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
February 2000,
Analytical chemistry,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
December 1984,
Cell biophysics,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
December 2015,
Lab on a chip,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
October 1999,
Journal of chromatography. B, Biomedical sciences and applications,
J P Andreux, and
A Merino, and
M Renard, and
F Forestier, and
P Cardot
April 2008,
The Analyst,
