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To assess the immunopathological significance of the increased replication of human T-cell leukemia virus type I (HTLV-I) in HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) we investigated the dynamics of HTLV-I proviral DNA in peripheral blood mononuclear cells (PBMC) of HAM/TSP patients at different clinical stages. We compared the dynamics to those of asymptomatic HTLV-I carriers (AC). The estimation of the amount of HTLV-I proviral DNA was carried out by quantitative polymerase chain reaction of serially diluted DNA samples where it was feasible to titrate 0.04-80 copies per 100 PBMC. The proviral DNA quantified in six patients with HAM/TSP was 2-20 copies per 100 PBMC, while that in eight cases of AC was 0.04-8 copies per 100 PBMC. Thus, the amount of HTLV-I proviral DNA in HAM/TSP patients was 3-50 times as high as that of AC. When we followed up HAM/TSP patients for 1-3 years, the amount of HTLV-I proviral DNA fluctuated from 4 to 10-fold. These data suggest that the rate of HTLV-I replication increases in HAM/TSP and the amount of HTLV-I proviral DNA fluctuates in their clinical course. Fluctuation in the amount of HTLV-I proviral DNA may reflect dynamics of HTLV-I infected cell proliferation and immunological suppression in vivo in HAM/TSP patients.
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
November 1988,
Journal of the neurological sciences,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
January 2003,
Blood cells, molecules & diseases,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
February 1993,
The American journal of tropical medicine and hygiene,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
February 1994,
Journal of acquired immune deficiency syndromes,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
February 1991,
Annals of neurology,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
January 1992,
Annals of neurology,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
July 2020,
PLoS neglected tropical diseases,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
August 1994,
Annals of neurology,
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
November 1991,
Presse medicale (Paris, France : 1983),
R Kubota, and
T Fujiyoshi, and
S Izumo, and
S Yashiki, and
I Maruyama, and
M Osame, and
S Sonoda
June 1992,
International journal of cancer,
