Biomaterial Database

Studies on activation and levels of haemolytic complement of buffalo (Bubalus bubalis). III. C3 haemolytic activity in health and chronic disease. 1993

S Singh, and M C Goel, and D P Monga

Department of Veterinary Microbiology, Haryana Agricultural University, Hisar, India.

Estimation of haemolytic complement component C3 activity in serum was done by using buffalo serum functionally depleted of C3, with methylamine. In the one-step alternate pathway (AP) haemolytic assay, C3 activity in the serum was estimated by its capacity to reconstitute the AP haemolytic activity in C3 depleted serum. Levels of haemolytic C3 were determined in the sera of 70 apparently healthy buffaloes and of seven diseased buffaloes of which five were suffering from Johne's disease (JD) and two from JD and tuberculosis (TB). The haemolytic C3 levels in healthy animals varied with age, reaching peak values in the 2.5-3-year-old, declining after 4 years of age. The C3 levels in buffaloes suffering from chronic diseases was significantly higher (P < 0.05) than the levels in healthy buffaloes of the same age group.

UI	MeSH Term	Description	Entries
D010283	Paratuberculosis	A chronic GASTROENTERITIS in RUMINANTS caused by MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS.	Johne's Disease,Johne Disease,Disease, Johne,Disease, Johne's,Johnes Disease,Paratuberculoses
D002020	Buffaloes	Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus.	Bubalus,Syncerus,Water Buffaloes,Buffalo,Water Buffalo,Buffalo, Water
D002908	Chronic Disease	Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care (Dictionary of Health Services Management, 2d ed). For epidemiological studies chronic disease often includes HEART DISEASES; STROKE; CANCER; and diabetes (DIABETES MELLITUS, TYPE 2).	Chronic Condition,Chronic Illness,Chronically Ill,Chronic Conditions,Chronic Diseases,Chronic Illnesses,Condition, Chronic,Disease, Chronic,Illness, Chronic
D003167	Complement Activation	The sequential activation of serum COMPLEMENT PROTEINS to create the COMPLEMENT MEMBRANE ATTACK COMPLEX. Factors initiating complement activation include ANTIGEN-ANTIBODY COMPLEXES, microbial ANTIGENS, or cell surface POLYSACCHARIDES.	Activation, Complement,Activations, Complement,Complement Activations
D003170	Complement Pathway, Alternative	Complement activation initiated by the interaction of microbial ANTIGENS with COMPLEMENT C3B. When COMPLEMENT FACTOR B binds to the membrane-bound C3b, COMPLEMENT FACTOR D cleaves it to form alternative C3 CONVERTASE (C3BBB) which, stabilized by COMPLEMENT FACTOR P, is able to cleave multiple COMPLEMENT C3 to form alternative C5 CONVERTASE (C3BBB3B) leading to cleavage of COMPLEMENT C5 and the assembly of COMPLEMENT MEMBRANE ATTACK COMPLEX.	Alternative Complement Pathway,Properdin Pathway,Alternative Complement Activation Pathway,Complement Activation Pathway, Alternative
D003176	Complement C3	A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.	C3 Complement,C3 Precursor,Complement 3,Complement C3 Precursor,Complement Component 3,Precursor-Complement 3,Pro-C3,Pro-Complement 3,C3 Precursor, Complement,C3, Complement,Complement, C3,Component 3, Complement,Precursor Complement 3,Precursor, C3,Precursor, Complement C3,Pro C3,Pro Complement 3
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014397	Tuberculosis, Pulmonary	MYCOBACTERIUM infections of the lung.	Pulmonary Consumption,Pulmonary Phthisis,Pulmonary Tuberculoses,Pulmonary Tuberculosis,Tuberculoses, Pulmonary,Consumption, Pulmonary,Consumptions, Pulmonary,Phthises, Pulmonary,Phthisis, Pulmonary,Pulmonary Consumptions,Pulmonary Phthises
D015941	Complement Hemolytic Activity Assay	A screening assay for circulating COMPLEMENT PROTEINS. Diluted SERUM samples are added to antibody-coated ERYTHROCYTES and the percentage of cell lysis is measured. The values are expressed by the so called CH50, in HEMOLYTIC COMPLEMENT units per milliliter, which is the dilution of serum required to lyse 50 percent of the erythrocytes in the assay.	CH50 Assay,Complement H50,Hemolytic Titration Assay,Serum Complement Titer,Total Hemolytic Complement,Whole Complement Titer,CH(50),Assay, CH50,Assay, Hemolytic Titration,Assays, CH50,Assays, Hemolytic Titration,CH50 Assays,Complement H50s,Complement, Total Hemolytic,Complements, Total Hemolytic,Hemolytic Complement, Total,Hemolytic Complements, Total,Hemolytic Titration Assays,Serum Complement Titers,Titer, Serum Complement,Titer, Whole Complement,Titers, Serum Complement,Titers, Whole Complement,Titration Assay, Hemolytic,Titration Assays, Hemolytic,Total Hemolytic Complements,Whole Complement Titers