Biomaterial Database

Development of storage granules during megakaryocyte maturation: accumulation of adenine nucleotides and the capacity for serotonin sequestration. 1993

C M Wojenski, and P K Schick

Cardeza Foundation for Hematologic Research, Philadelphia, PA 19107-5099.

The specific release of platelet-dense body constituents such as adenine nucleotides and serotonin has a fundamental role in hemostasis. The content of adenine nucleotides in platelet dense bodies is probably established in megakaryocytes, but very little is known about this process. To gain a better understanding of platelet development, we studied the storage and metabolic pools of adenine nucleotides and the capacity for serotonin sequestration in storage granules during megakaryocyte maturation. Megakaryocytes were isolated from guinea pig bone marrow and separated in subgroups at different phases of maturation. The sequestration of adenine nucleotides and serotonin in storage granules was assessed by using calcium ionophore to induce secretion under nonlytic conditions, and metabolic pool adenine nucleotides were evaluated by using digitonin under controlled lysis conditions. The study showed that there were similar amounts of cytoplasmic adenosine triphosphate (ATP) in the mature and immature fractions based on digitonin-induced controlled lysis (1.26 +/- 0.37 nmol/microgram phosphorus vs 1.23 +/- 0.44 nmol/microgram phosphorus). However, only the mature cells contained a significant amount of storage pool ATP (0.41 +/- 0.19 nmol/microgram phosphorus vs 0.05 +/- 0.05 nmol/microgram phosphorus) released in response to A23187. The subgroups of megakaryocytes contained equal amounts of total adenosine diphosphate (ADP) extractable with ethanol (0.49 +/- 0.14 nmol/microgram phosphorus vs 0.55 +/- 0.50 nmol/microgram phosphorus) but only mature cells contained ADP in the storage granules (0.25 +/- 0.13 nmol/microgram phosphorus vs 0.07 +/- 0.05 nmol/microgram phosphorus).(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D007099	Imipramine	The prototypical tricyclic antidepressant. It has been used in major depression, dysthymia, bipolar depression, attention-deficit disorders, agoraphobia, and panic disorders. It has less sedative effect than some other members of this therapeutic group.	Imidobenzyle,Imizin,4,4'-Methylenebis(3-hydroxy-2-naphthoic acid)-3-(10,11-dihydro-5H-dibenzo(b,f)azepin-5-yl)-N,N-dimethyl-1-propanamine (1:2),Imipramine Hydrochloride,Imipramine Monohydrochloride,Imipramine Pamoate,Janimine,Melipramine,Norchlorimipramine,Pryleugan,Tofranil
D007770	L-Lactate Dehydrogenase	A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.	Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D008533	Megakaryocytes	Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.	Megakaryocyte
D001792	Blood Platelets	Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.	Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D004072	Digitonin	A glycoside obtained from Digitalis purpurea; the aglycone is digitogenin which is bound to five sugars. Digitonin solubilizes lipids, especially in membranes and is used as a tool in cellular biochemistry, and reagent for precipitating cholesterol. It has no cardiac effects.	Digitin
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000001	Calcimycin	An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.	4-Benzoxazolecarboxylic acid, 5-(methylamino)-2-((3,9,11-trimethyl-8-(1-methyl-2-oxo-2-(1H-pyrrol-2-yl)ethyl)-1,7-dioxaspiro(5.5)undec-2-yl)methyl)-, (6S-(6alpha(2S,3S),8beta(R*),9beta,11alpha))-,A-23187,A23187,Antibiotic A23187,A 23187,A23187, Antibiotic
D000227	Adenine Nucleotides		Adenine Nucleotide,Adenosine Phosphate,Adenosine Phosphates,Nucleotide, Adenine,Nucleotides, Adenine,Phosphate, Adenosine,Phosphates, Adenosine
D000244	Adenosine Diphosphate	Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.	ADP,Adenosine Pyrophosphate,Magnesium ADP,MgADP,Adenosine 5'-Pyrophosphate,5'-Pyrophosphate, Adenosine,ADP, Magnesium,Adenosine 5' Pyrophosphate,Diphosphate, Adenosine,Pyrophosphate, Adenosine
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia