-transparent.png){kind=logo}
The existence of dopamine receptor subtypes outside the central nervous system has been suggested by pharmacological and biochemical techniques. Whether the renal dopamine receptors are distinct from those cloned from the brain remains to be determined. Indeed, the expression of any of these receptor genes in the kidney has not been demonstrated definitively. In this study, we amplified D1A receptor cDNA from microdissected proximal convoluted tubules of the rat kidney by reverse transcription-polymerase chain reaction; primers were based on the sequence of rat D1A cDNA cloned from the brain and corresponded to the third cytoplasmic loop of the receptor. Specificity of the amplified products was verified by restriction analysis, Southern blots, and sequencing. Furthermore, solution hybridization indicated the presence of a single ribonuclease-protected RNA species corresponding to the D1A receptor mRNA in proximal tubules. These studies report for the first time the presence of D1A receptor message in the kidney.
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
June 1995,
The American journal of physiology,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
January 1997,
Clinical and experimental hypertension (New York, N.Y. : 1993),
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
April 1996,
Methods and findings in experimental and clinical pharmacology,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
January 2015,
Hypertension (Dallas, Tex. : 1979),
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
September 2006,
Circulation research,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
June 2012,
Hypertension research : official journal of the Japanese Society of Hypertension,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
October 1999,
Molecular and cellular endocrinology,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
November 1993,
The Journal of biological chemistry,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
September 1996,
DNA and cell biology,
I Yamaguchi, and
P A Jose, and
M M Mouradian, and
L M Canessa, and
F J Monsma, and
D R Sibley, and
K Takeyasu, and
R A Felder
July 2017,
Hypertension research : official journal of the Japanese Society of Hypertension,
