BIOMAT Database Logo BIOMAT Database Logo

A corrected primary structure for dog-fish Scylliorhinus caniculus protamine Z3. 1993

M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
URA 409 CNRS, Université de Lille II, Institut de Recherches sur le Cancer, France.

We have redetermined the primary structure for dog-fish protamine using automated amino-acid sequencing associated to mass spectrometry techniques and report, on the basis of these findings, that the previously published amino-acid sequence is incorrect. The correct protamine sequence is 37 amino acids long and differs from the original published sequence by the C-terminal hexapeptide Arg32-Gly-Arg-Arg-Ser-Arg37.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011479 Protamines A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692) Protamine,Protamine Sulfate,Protamine Chloride,Chloride, Protamine,Sulfate, Protamine
D002918 Chymotrypsin A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side. Alpha-Chymotrypsin Choay,Alphacutanée,Avazyme
D004284 Dogfish Sharks of the family Squalidae, also called dogfish sharks. They comprise at least eight genera and 44 species. Their LIVER is valued for its oil and its flesh is often made into fertilizer. Squalidae,Dogfishes
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass
D014357 Trypsin A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4. Tripcellim,Trypure,beta-Trypsin,beta Trypsin

Related Publications

M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Primary structure of the protamine isolated from the sperm nuclei of the dog-fish Scylliorhinus caniculus.
October 1981, European journal of biochemistry,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Isolation and characterization of a cDNA clone encoding testis protamine Z1 from the dog-fish Scylliorhinus caniculus.
June 1987, European journal of biochemistry,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Nucleotide sequence of a cDNA clone encoding Scylliorhinus caniculus protamine Z2.
October 1986, European journal of biochemistry,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Nuclear basic protein transition during sperm differentiation. Primary structure of the spermatid-specific protein S2 from the dog-fish Scylliorhinus caniculus.
March 1989, European journal of biochemistry,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Extraction, purification and characterization of the sperm protamines of the dog-fish Scylliorhinus caniculus.
October 1983, Biochimica et biophysica acta,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Purification and characterization of two basic spermatid-specific proteins isolated from the dog-fish Scylliorhinus caniculus.
February 1983, FEBS letters,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
Nuclear basic protein transition during sperm differentiation. Amino acid sequence of a spermatid-specific protein from the dog-fish Scylliorhinus caniculus.
November 1987, European journal of biochemistry,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
A cytological study of the neurohypophysis of Scylliorhinus caniculus.
January 1955, Zeitschrift fur Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948),
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
[Development of the paraphysis cerebri in Scylliorhinus caniculus].
October 1956, Nederlands tijdschrift voor geneeskunde,
M Kouach, and M Jaquinod, and D Belaïche, and P Sautière, and A van Dorsselaer, and P Chevaillier, and G Briand
A corrected primary sequence for bull protamine.
July 1986, Biochimica et biophysica acta,
Copied contents to your clipboard!