The purpose of this investigation was to determine the effects of increasing specific (rowing ergometer) and non-specific (cycle ergometer) workloads on parameters relating to the ventilatory threshold (Tvent) and work efficiency. When highly trained male rowers were tested using non-specific workloads, their %VO2 max values at Tvent were close to those characteristic of untrained subjects (74.6 +/- 6.2% VO2 max). However, when we tested the same subjects using specific workloads, we recorded values typical of highly trained athletes (85.0 +/- 4.4% VO2 max). For the non-specific exercise on the cycle ergometer, we recorded work efficiency values close to those of untrained subjects (22.8 +/- 2.1%); however, for the specific exercise on the rowing ergometer, we recorded much lower values (16.4 +/- 3.1%). Because of the results of the non-specific submaximal exercise tests, we suggest caution in the interpretation of physiological variables that may be sensitive to training status. The evaluation of Tvent and work efficiency as supplementary parameters during laboratory studies will enable researchers to ascertain the effectiveness of the training process used, as well as indicating the specificity of the loading apparatus.