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An enzyme that catalyzes the hydrolysis of both glutamine and asparagine has been purified to homogeneity from extracts of Pseudomonas acidovorans. The enzyme having a ratio of glutaminase to asparaginase of 1.45:1.0 can be purified by a relatively simple procedure and is stable upon storage. The glutaminase-asparaginase has a relatively high affinity for L-asparagine (Km=1.5 X 10(-5) M) and L-glutamine (Km=2.2 X 10(-5) M) and has a molecular weight of approximately 156,000 the subunit molecular weight being approximately 39,000. Injections of the enzyme produced only slight increases in the survival time of C3H/HE mice carrying the asparagine-requiring 6C2HED Gardner lymphoma and of white Swiss mice carrying the glutamine-requiring Ehrlich lymphoma.
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
December 1972,
Biochimica et biophysica acta,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
April 1976,
The Journal of biological chemistry,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
January 1976,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite naturwissenschaftliche Abt.: Allgemeine, landwirtschaftliche und technische Mikrobiologie,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
September 1976,
The Journal of biological chemistry,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
November 1975,
Biochimica et biophysica acta,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
January 1975,
Voprosy meditsinskoi khimii,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
October 1964,
Archives of biochemistry and biophysics,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
May 1977,
Journal of molecular biology,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
February 1972,
Biochemical and biophysical research communications,
L Davidson, and
D R Brear, and
P Wingard, and
J Hawkins, and
G B Kitto
January 2011,
Bioresource technology,
