Preparation and initial characterization of crystals of the photoprotein aequorin from Aequorea victoria. 1993

L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
Naval Research Laboratory, Washington, D.C. 20375.

Crystals of recombinant aequorin, the photoprotein from the jellyfish Aequorea victoria, have been grown from solutions containing sodium phosphate. The crystals grow as thin plates which diffract to beyond 2.2 A resolution. The crystals are orthorhombic, space group P2(1)2(1)2(1); the axes are a = 89.1(1), b = 88.4(1), and c = 52.7(1) A. The asymmetric unit contains two molecules. Crystals exposed to calcium ion solutions emit a steady glow and slowly deteriorate, confirming that the crystals consist of a charged, competent photoprotein. This represents the first successful preparation of single crystals of a photoprotein suitable for diffraction analysis.

UI MeSH Term Description Entries
D008163 Luminescent Measurements Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE. Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D000331 Aequorin A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350). Aequorine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions
D037401 Scyphozoa The class of true jellyfish, in the phylum CNIDARIA. They are mostly free-swimming marine organisms that go through five stages in their life cycle and exhibit two body forms: polyp and medusa. Aurelia aurita,Chrysaora fuscescens,Jellyfish, Moon,Jellyfish, True,Moon Jellyfish,Sea Nettle, West Coast,Moon Jelly,Sea Nettle, Pacific,Aurelia auritas,Chrysaora fuscescen,Jellies, Moon,Jelly, Moon,Jellyfishs, Moon,Jellyfishs, True,Moon Jellies,Moon Jellyfishs,Nettle, Pacific Sea,Nettles, Pacific Sea,Pacific Sea Nettle,Pacific Sea Nettles,Scyphozoas,Sea Nettles, Pacific,True Jellyfish,True Jellyfishs,auritas, Aurelia,fuscescens, Chrysaora

Related Publications

L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
March 1981, Analytical biochemistry,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
July 1975, Nature,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
October 1971, Biochemistry,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
March 2001, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
September 1988, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
September 2022, Photochemistry and photobiology,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
February 1982, FEBS letters,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
November 1986, Proceedings of the National Academy of Sciences of the United States of America,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
November 1985, Biochemistry,
L I Hannick, and D C Prasher, and L W Schultz, and J R Deschamps, and K B Ward
June 1978, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!