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Distribution of TaqI sites along human chromosomes revealed by in situ enzyme-nick translation. 1993
Departamento de Biología, Unidad de Genética, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
We have studied the relative richness of TaqI sites along human chromosomes by means of a nonradioactive in situ enzyme-nick translation procedure. Regions with a higher content of these sequences are shown to be the noncentromeric heterochromatin blocks, whereas within euchromatin, terminal R-bands are the domains more enriched in these sites. Results obtained suggest that the method of performing enzyme digestions using time as a variable, and then in situ nick translation, provides much more complete information about the distribution of enzyme sequences along chromosomes than standard enzyme digestions.
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