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Selective extraction of sulfate conjugates of basic drugs from biological matrices has been difficult because of their highly polar nature. Immunoaffinity isolation may be the best solution to this analytical problem. This was tested for a model compound, the metabolite 4'-hydroxypropranolol sulfate (HOPS), which was effectively extracted from plasma by a column containing antibodies to the parent drug propranolol. The specificity was very high, giving little interference from the biological material in subsequent high-performance liquid chromatographic analysis with fluorometric detection. The method for HOPS was highly reproducible and provided a sensitivity of 1 ng/ml plasma. The technique was applied to measurements of HOPS in plasma after therapeutic doses of propranolol as well as to the individual enantiomers after chiral derivatization.
T D Eller, and
U K Walle, and
T Walle
January 1984,
Journal of chromatography,
T D Eller, and
U K Walle, and
T Walle
January 1985,
Drug metabolism and disposition: the biological fate of chemicals,
T D Eller, and
U K Walle, and
T Walle
January 1992,
Drug metabolism and disposition: the biological fate of chemicals,
T D Eller, and
U K Walle, and
T Walle
January 1991,
Drug metabolism and disposition: the biological fate of chemicals,
T D Eller, and
U K Walle, and
T Walle
April 1979,
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T D Eller, and
U K Walle, and
T Walle
January 1991,
Drug metabolism and disposition: the biological fate of chemicals,
T D Eller, and
U K Walle, and
T Walle
June 1985,
Journal of medicinal chemistry,
T D Eller, and
U K Walle, and
T Walle
January 1985,
Comparative biochemistry and physiology. B, Comparative biochemistry,
T D Eller, and
U K Walle, and
T Walle
September 1978,
Circulation,
T D Eller, and
U K Walle, and
T Walle
June 1981,
Journal of pharmaceutical sciences,
