Biomaterial Database

Indomethacin-induced gastrointestinal ulcers in rats: effects of dietary fatty acids and endotoxin. 1993

J J Turek, and I A Schoenlein

Purdue University, School of Veterinary Medicine, Department of Veterinary Anatomy, W. Lafayette, IN 47907.

The purpose of this study was to determine how dietary n-3 and n-6 polyunsaturated fatty acids (PUFA) affected the gastrointestinal response to lipopolysaccharide (LPS) and indomethacin (INDO) in the rat. Rats were fed diets containing 12.5% linseed oil (LO-enriched in short-chain n-3 PUFA) or corn oil (CO-enriched in n-6 PUFA). After 30 days on the diets, rats were given one of three treatments 1:10 mg/kg O55:B5 Escherichia coli LPS intraperitoneally (i.p.), 2:25 mg/kg INDO subcutaneously (s.c.), 3: a combination of 10 mg/kg i.p. LPS and 25 mg/kg (s.c.) INDO given 30 min before LPS. 20 h after challenge, rats were given an intravenous injection of Monastral Blue B to stain ulcer areas in the gastrointestinal tract. Lipopolysaccharide did not result in any Monastral Blue B vascular leakage in the gastrointestinal tract. Rats on the LO diet had significantly increased stomach and intestinal ulcers compared to CO fed rats. When rats were challenged with LPS and INDO, the LPS almost completely eliminated small intestinal ulcers, but enhanced ulcer development in the stomach.

UI	MeSH Term	Description	Entries
D007213	Indomethacin	A non-steroidal anti-inflammatory agent (NSAID) that inhibits CYCLOOXYGENASE, which is necessary for the formation of PROSTAGLANDINS and other AUTACOIDS. It also inhibits the motility of POLYMORPHONUCLEAR LEUKOCYTES.	Amuno,Indocid,Indocin,Indomet 140,Indometacin,Indomethacin Hydrochloride,Metindol,Osmosin
D008043	Linseed Oil	The fixed oil obtained from the dried ripe seed of linseed, Linum usitatissimum (L. Linaceae). It is used as an emollient in liniments, pastes, and medicinal soaps, and in veterinary medicine as a laxative. It is also called flaxseed oil. (Dorland, 28th ed)	Flaxseed Oil,Oil, Flaxseed,Oil, Linseed
D008070	Lipopolysaccharides	Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)	Lipopolysaccharide,Lipoglycans
D008297	Male		Males
D003314	Corn Oil	Oil from ZEA MAYS or corn plant.	Maize Oil,Lipomul,Corn Oils,Lipomuls,Maize Oils,Oil, Corn,Oil, Maize,Oils, Corn,Oils, Maize
D004042	Dietary Fats, Unsaturated	Unsaturated fats or oils used in foods or as a food.	Dietary Oils,Unsaturated Dietary Fats,Dietary Fat, Unsaturated,Dietary Oil,Fat, Unsaturated Dietary,Fats, Unsaturated Dietary,Oil, Dietary,Oils, Dietary,Unsaturated Dietary Fat
D004731	Endotoxins	Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.	Endotoxin
D005227	Fatty Acids	Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)	Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D005767	Gastrointestinal Diseases	Diseases in any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM.	Cholera Infantum,Gastrointestinal Disorders,Functional Gastrointestinal Disorders,Gastrointestinal Disorders, Functional,Disease, Gastrointestinal,Diseases, Gastrointestinal,Functional Gastrointestinal Disorder,Gastrointestinal Disease,Gastrointestinal Disorder,Gastrointestinal Disorder, Functional
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia