BIOMAT Database Logo BIOMAT Database Logo

Overproduction of proteins using expression-cassette polymerase chain reaction. 1993

K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009838 Oligodeoxyribonucleotides A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties. Oligodeoxynucleotide,Oligodeoxyribonucleotide,Oligodeoxynucleotides
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003062 Codon A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE). Codon, Sense,Sense Codon,Codons,Codons, Sense,Sense Codons
D004274 DNA, Recombinant Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Overproduction and dissection of proteins by the expression-cassette polymerase chain reaction.
March 1990, Proceedings of the National Academy of Sciences of the United States of America,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
[Quantification of gene expression using the polymerase chain reaction].
January 2002, Vojnosanitetski pregled,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Efficient production of active and mutated ADP-ribosyltransferase (S1) of pertussis toxin using affinity expression cassette polymerase chain reaction.
March 1994, FEMS immunology and medical microbiology,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Expression profiling of tubulin isotypes and microtubule-interacting proteins using real-time polymerase chain reaction.
January 2010, Methods in cell biology,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Using oligonucleotides for cloning of Rab proteins by polymerase chain reaction.
January 1995, Methods in enzymology,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction.
May 1990, Nucleic acids research,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Assessment of glucose transporter gene expression using the polymerase chain reaction.
May 1991, Endocrinology,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Efficient complementary DNA amplification and expression using polymerase chain reaction technology.
January 1993, Methods in enzymology,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Immunopolymerase chain reaction using real-time polymerase chain reaction for detection.
June 2000, Analytical biochemistry,
K D MacFerrin, and L Chen, and M P Terranova, and S L Schreiber, and G L Verdine
Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer.
June 2000, Nucleic acids research,
Copied contents to your clipboard!