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Germinated spores of Actinomadura verrucosospora subsp. neohibisca E-40, a high pradimicins producer, were mutagenized by N-methyl-N'-nitro-N-nitrosoguanidine and/or UV treatment. Thirty-seven mutants which did not produce pradimicin were selected to test for cosynthesis ability, and classified into nine classes. On the basis of their cosynthesis ability and bioconversion results, we concluded that strain JN-213 (class III) was a true converter and that strains JN-219 (class IV), JN-47 (class V) and JNU-46 (class VI) were secretors accumulating biosynthetic intermediates of pradimicin, and that strains JN-59 (class VII), JN-58 (class VIII) and JN-207 (class IX) were producers of shunt metabolites of pradimicin biosynthesis. TLC and HPLC analyses of the fermentation broths of individual strains showed that 8 new compounds were produced along with pradinone I, pradimicinone I, 11-O-demethylpradimicinone II and 7-O-methylpradimicinone II.
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
January 1981,
Folia microbiologica,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
January 1977,
Genetika,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
June 2009,
Chembiochem : a European journal of chemical biology,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
July 1992,
The Journal of antibiotics,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
February 1997,
Expert opinion on investigational drugs,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
August 1986,
Journal of bacteriology,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
April 1993,
The Journal of antibiotics,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
January 2000,
Methods in enzymology,
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
January 1999,
Methods in molecular biology (Clifton, N.J.),
T Furumai, and
S Kakinuma, and
H Yamamoto, and
N Komiyama, and
K Suzuki, and
K Saitoh, and
T Oki
November 1988,
The Journal of antibiotics,
