-transparent.png){kind=logo}
Isoelectric focusing of human orosomucoid (ORM) was studied following different sample treatment. It is shown that: (i) alkylation with iodoacetamide leads to a drastic change in the isoelectric point (pI) of both ORM1 F2 and ORM2 A gene products and greatly improves the discrimination between ORM1 F1 and ORM1 F2; (ii) previous reduction of the molecule with dithiothreitol partially inhibits the pI transitions with resultant artifactual ORM1 F1F2S patterns that correspond in most cases to F2S phenotypes. With the technique now described, the persistence of three ORM1 gene products was found in only one individual and the segregation analysis is consistent with the existence of a rare ORM1*F2S haplotype.
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
August 1988,
Electrophoresis,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
March 1997,
Human genetics,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
August 1988,
American journal of human genetics,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
November 1987,
Human genetics,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
January 2000,
International journal of legal medicine,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
March 1998,
Journal of forensic sciences,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
January 1989,
Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
October 1986,
Human genetics,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
April 1996,
Human biology,
J Rocha, and
A Amorim, and
C Luckenbach, and
J Kömpf, and
H Ritter
January 1990,
Vox sanguinis,
