Biomaterial Database

Histochemical localization of glycosaminoglycans in the omasal papillae of sheep. 1993

Y Yamamoto, and N Kitamura, and J Yamada, and T Yamashita

Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.

Histochemical localization of the glycosaminoglycans in the omasal laminae were examined at light and electron microscopic levels. The core region of the omasal papillae was stained strongly with alcian blue at pH 2.5 and 1.0. The staining was degraded moderately and completely when tissue sections were pretreated with mild and active methylation, respectively. Alcianophilia was moderately decreased with saponification. Furthermore, enzymatic digestion procedures showed that these alcianophilic substances contained a large amount of hyaluronic acid and chondroitin sulphate. Ultrastructurally, a spider web-like structure was widely distributed among the spindle-shaped fibroblast-like cells and fibrous networks of collagen and elastin. These results suggest that the core region of the omasal papillae not only acts as a physical buffer resisting the local pressure from the lumen, but may also influence material transport through the omasal mucosa.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009851	Omasum	The third stomach of ruminants, situated on the right side of the abdomen at a higher level than the fourth stomach and between this latter and the second stomach, with both of which it communicates. From its inner surface project large numbers of leaves or folia, each of which possesses roughened surfaces. In the center of each folium is a band of muscle fibers which produces a rasping movement of the leaf when it contracts. One leaf rubs against those on either side of it, and large particles of food material are ground down between the rough surfaces, preparatory to further digestion in the succeeding parts of the alimentary canal. (Black's Veterinary Dictionary, 17th ed)	Omasums
D005260	Female		Females
D006025	Glycosaminoglycans	Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE).	Glycosaminoglycan,Mucopolysaccharides
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli