BIOMAT Database Logo BIOMAT Database Logo

Epidermal growth factor and insulin-like growth factor I enhance keratinocyte migration. 1993

Y Ando, and P J Jensen
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia 19104-6142.

Although their mechanisms of action are unclear, a number of growth factors has been shown to promote cutaneous wound repair. Keratinocyte migration and proliferation are required for re-epithelialization, and there is evidence to suggest that these processes may be regulated by one or more growth factors that promote wound repair. Using the phagokinetic assay, which allows direct observation of migration path as a gold-particle-free area, we examined the effects of epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) on human keratinocyte migration. Addition of EGF to defined medium in the absence of any other growth factor induced an increase in migration of 2.5-4.5 fold after overnight incubation; the effect of EGF on migration was concentration dependent, with a maximum at 10 to 50 ng/ml EGF. Concentration-dependent enhancement of keratinocyte migration was similarly observed with IGF-I as well as insulin. With all factors, migration was observed on colloidal gold plates coated with collagen IV or with fibronectin but not in the absence of matrix coating. To examine further the involvement of the EGF receptor in keratinocyte migration, we tested the effect of a monoclonal antibody to the EGF receptor that acts as an antagonist. EGF-induced migration was completely prevented by this antibody; however, the enhancement by insulin or IGF-I was not blocked. These results suggest that IGF-I and insulin enhance keratinocyte migration by a mechanism distinct from that of EGF.

UI MeSH Term Description Entries
D007328 Insulin A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1). Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D007334 Insulin-Like Growth Factor I A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor. IGF-I,Somatomedin C,IGF-1,IGF-I-SmC,Insulin Like Growth Factor I,Insulin-Like Somatomedin Peptide I,Insulin Like Somatomedin Peptide I
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003513 Cycloheximide Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis. Actidione,Cicloheximide
D004815 Epidermal Growth Factor A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form. EGF,Epidermal Growth Factor-Urogastrone,Urogastrone,Human Urinary Gastric Inhibitor,beta-Urogastrone,Growth Factor, Epidermal,Growth Factor-Urogastrone, Epidermal,beta Urogastrone
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D015603 Keratinocytes Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell. Keratinocyte

Related Publications

Y Ando, and P J Jensen
Insulin-like growth factor-I and epidermal growth factor regulate insulin-like growth factor binding protein-3 (IGFBP-3) in the human keratinocyte cell line HaCaT.
October 1995, The Journal of investigative dermatology,
Y Ando, and P J Jensen
Epidermal growth factor and keratinocyte growth factor differentially regulate epidermal migration, growth, and differentiation.
January 2000, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society,
Y Ando, and P J Jensen
Androgen receptor activation in prostatic tumor cell lines by insulin-like growth factor-I, keratinocyte growth factor, and epidermal growth factor.
October 1994, Cancer research,
Y Ando, and P J Jensen
Androgen receptor activation in prostatic tumor cell lines by insulin-like growth factor-I, keratinocyte growth factor and epidermal growth factor.
January 1995, European urology,
Y Ando, and P J Jensen
Gq-coupled purinergic receptors inhibit insulin-like growth factor-I/phosphoinositide 3-kinase pathway-dependent keratinocyte migration.
March 2010, Molecular biology of the cell,
Y Ando, and P J Jensen
Suppression of insulin-like growth factor I during epidermal growth factor-induced growth retardation.
March 1991, The American journal of physiology,
Y Ando, and P J Jensen
Renal epidermal growth factor and insulin-like growth factor I in acute renal failure.
January 1994, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
Y Ando, and P J Jensen
Characterization of insulin-like growth factor I and epidermal growth factor receptors in meningioma.
October 1989, Journal of neurosurgery,
Y Ando, and P J Jensen
Insulin-like growth factor I and truncated keratinocyte growth factor accelerate healing of left-sided colonic anastomoses.
January 2001, The British journal of surgery,
Y Ando, and P J Jensen
Epidermal growth factor and insulin-like growth factor I upregulate the expression of the epidermal growth factor system in rat liver.
April 2000, Journal of hepatology,
Copied contents to your clipboard!