Biomaterial Database

Production of heterogeneous carbohydrate-binding proteins by the host snail Biomphalaria glabrata following exposure to Echinostoma paraensei and Schistosoma mansoni. 1993

F P Monroy, and E S Loker

Department of Biology, University of New Mexico, Albuquerque 87131.

Hemolymph lectins may play an important role in the internal defense responses of gastropods to parasites. Two groups of known carbohydrate-binding polypeptides, of 150-220 kDa (designated as group 1 molecules, or G1M) and of 75-130 kDa (group 2 molecules, or G2M), were harvested from pooled plasma samples of Biomphalaria glabrata using affinity chromatography and examined using 2-dimensional electrophoresis. Plasma samples were derived from control snails or snails exposed 8 days earlier to the trematodes Echinostoma paraensei or Schistosoma mansoni. Plasma of control and S. mansoni-exposed snails contained little or no G1M, whereas plasma from E. paraensei-infected snails contained G1M covering a broad pI spectrum. G2M resolved as 1-2 isoforms in control plasma and up to 4 relatively faint isoforms in plasma from S. mansoni-exposed snails, and as 5-6 resolvable isoforms in plasma from E. paraensei-infected snails. Plasma from individual snails contained as many as 5 G2M polypeptides following exposure to E. paraensei. Exposure to trematode larvae stimulated production by B. glabrata of increased abundance and diversity of carbohydrate-binding proteins. The 2 trematode species provoked different responses, and 2 B. glabrata strains studied (M line and 13-16-R1 strains) differed from one another in their responses.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D004449	Echinostoma	A genus of intestinal flukes of the family Echinostomatidae which consists of many species. They occur in man and other vertebrates. The intermediate hosts are frequently mollusks.	Echinostoma ilocanum,Euparyphium ilocanum,Fascioletta ilocanum,Echinostoma ilocanums,Echinostomas,Euparyphium ilocanums,Fascioletta ilocanums,ilocanum, Echinostoma,ilocanum, Fascioletta,ilocanums, Euparyphium
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D006458	Hemolymph	The blood/lymphlike nutrient fluid of some invertebrates.	Hemolymphs
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001700	Biomphalaria	A genus of planorbid freshwater snails, species of which are intermediate hosts of Schistosoma mansoni.	Australorbis,Australorbis glabratus,Australorbi,Biomphalarias
D012550	Schistosoma mansoni	A species of trematode blood flukes of the family Schistosomatidae. It is common in the Nile delta. The intermediate host is the planorbid snail. This parasite causes schistosomiasis mansoni and intestinal bilharziasis.	Schistosoma mansonus,mansonus, Schistosoma
D015180	Electrophoresis, Gel, Two-Dimensional	Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.	Gel Electrophoresis, Two-Dimensional,Polyacrylamide Gel Electrophoresis, Two-Dimensional,2-D Gel Electrophoresis,2-D Polyacrylamide Gel Electrophoresis,2D Gel Electrophoresis,2D PAGE,2D Polyacrylamide Gel Electrophoresis,Electrophoresis, Gel, 2-D,Electrophoresis, Gel, 2D,Electrophoresis, Gel, Two Dimensional,Polyacrylamide Gel Electrophoresis, 2-D,Polyacrylamide Gel Electrophoresis, 2D,Two Dimensional Gel Electrophoresis,2 D Gel Electrophoresis,2 D Polyacrylamide Gel Electrophoresis,Electrophoresis, 2-D Gel,Electrophoresis, 2D Gel,Electrophoresis, Two-Dimensional Gel,Gel Electrophoresis, 2-D,Gel Electrophoresis, 2D,Gel Electrophoresis, Two Dimensional,PAGE, 2D,Polyacrylamide Gel Electrophoresis, 2 D,Polyacrylamide Gel Electrophoresis, Two Dimensional,Two-Dimensional Gel Electrophoresis
D037102	Lectins	Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.	Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal