BIOMAT Database Logo BIOMAT Database Logo

Sialic acids in human gastric aspirates: detection of 9-O-lactyl- and 9-O-acetyl-N-acetylneuraminic acids and a decrease in total sialic acid concentration with age. 1993

A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
University Department of Medicine, Bristol Royal Infirmary, U.K.

1. The total sialic acid content of human gastric aspirates was measured using a colorimetric assay. Care was taken to optimize the assay and to eliminate interference. 2. The sialic acid content of gastric aspirates collected under resting conditions from 77 patients with non-ulcer dyspepsia was found to decrease with age from > 100 micrograms/ml at 25 years and younger to < 20 micrograms/ml above 70 years of age. 3. Analysis of the sialic acids by gas chromatography, mass spectrometry and thin-layer chromatography showed the presence of N-acetylneuraminic acid and two O-acylated derivatives, 9-O-acetyl- and 9-O-lactyl-N-acetylneuraminic acids. These forms were predominantly glycosidically bound. 4. Thin-layer chromatographic analysis of individual aspirate samples showed that the O-acetylated sialic acids were present in all samples, with a maximum of 25% and a minimum of 5% of the total sialic acids.

UI MeSH Term Description Entries
D008297 Male Males
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D005260 Female Females
D005750 Gastric Juice The liquid secretion of the stomach mucosa consisting of hydrochloric acid (GASTRIC ACID); PEPSINOGENS; INTRINSIC FACTOR; GASTRIN; MUCUS; and the bicarbonate ion (BICARBONATES). (From Best & Taylor's Physiological Basis of Medical Practice, 12th ed, p651) Gastric Juices,Juice, Gastric,Juices, Gastric
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly
D000369 Aged, 80 and over Persons 80 years of age and older. Oldest Old

Related Publications

A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Identification by gas-liquid chromatography-mass spectrometry of 4-O-acetyl-9-O-lactyl-N-acetyl-neuraminic acid, a new sialic acid from horse submandibular gland.
June 1980, Biochimica et biophysica acta,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Identification of 9-O-acetyl-N-acetylneuraminic acid (Neu5,9Ac2) as main O-acetylated sialic acid species of GD2 in breast cancer cells.
February 2019, Glycoconjugate journal,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Migration of O-acetyl groups in N,O-acetylneuraminic acids.
February 1987, European journal of biochemistry,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
New sialic acids. Identification of N-glycoloyl-O-acetylneuraminic acids and N-acetyl-O-glycoloylneuraminic acids by improved methods for detection of N-acyl and O-acyl groups and by gas-liquid chromatography.
December 1974, European journal of biochemistry,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
SIALIC ACID (N-ACETYLNEURAMINIC ACID) IN HUMAN SERUM.
November 1964, Clinical chemistry,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Synthesis of 9-O-acyl- and 4-O-acetyl-sialic acids.
September 1987, Carbohydrate research,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Sialic acids (N,7-O-diacetylneuraminic acid and N-acetylneuraminic acid) in Escherichia coli. I. Isolation and identification.
December 1961, Journal of bacteriology,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Synthesis of N-acetyl-9-O-acetylneuraminic acid alpha-p-amino-phenylthioketoside and its application as ligand in the affinity chromatography of a lectin with preferential affinity to O-acetylated sialic acids.
December 1992, Biological chemistry Hoppe-Seyler,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Quantitative Standards of 4-O-Acetyl- and 9-O-Acetyl-N-Acetylneuraminic Acid for the Analysis of Plasma and Serum.
March 2022, Chembiochem : a European journal of chemical biology,
A P Corfield, and S A Wagner, and A Safe, and R A Mountford, and J R Clamp, and J P Kamerling, and J F Vliegenthart, and R Schauer
Metabolism of 4-O-methyl-N-acetylneuraminic acid a synthetic sialic acid.
May 1980, European journal of biochemistry,
Copied contents to your clipboard!