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Visualization of ultraviolet light-induced thymine dimers in DNA by immunoelectron microscopy. 1993
M U Udono, and
M Hori, and
M Hirayama, and
H Yoshida, and
T Suematsu, and
T Suematsu, and
T Mori, and
T Matsunaga, and
T Mizuno, and
O Nikaido
Department of Dermatology, Nagasaki University School of Medicine, Japan.
To see the damage of DNA due to ultraviolet-B more distinctly, immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. As a result, we could detect the existence of thymine dimers on human genomic DNA and pUC18 plasmid DNA visually. This technique can be useful to locate the photoproducts formed on DNA.
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