Capacity of rete testicular and cauda epididymal boar spermatozoa to undergo the acrosome reaction and subsequent fusion with egg plasma membrane. 1993

H Harayama, and H Kusunoki, and S Kato
Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Japan.

The capacity to undergo the acrosome reaction and subsequent fusion with egg plasma membrane was examined in rete testicular and cauda epididymal spermatozoa from boars. Sperm penetration assay using zona-free hamster eggs demonstrated that the penetration rates for rete testicular spermatozoa preincubated for induction of the acrosome reaction for 2 and 3 h were 55% and 97%, respectively. However, most of the eggs (93%) were penetrated with polyspermy by cauda epididymal cells preincubated for 2 h. Results obtained by the triple-stain technique revealed the percentages of acrosome-reacted spermatozoa in the rete testicular and cauda epididymal samples preincubated for 3 h to be 61% and 74%, respectively. These results indicate that many rete testicular spermatozoa possess the capacity to undergo the acrosome reaction and subsequent fusion with egg plasma membrane in vitro, which appears to be completely established only after sperm transit through at least the proximal part of the epididymis.

UI MeSH Term Description Entries
D008297 Male Males
D008561 Membrane Fusion The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes. Fusion, Membrane,Fusions, Membrane,Membrane Fusions
D008647 Mesocricetus A genus in the order Rodentia and family Cricetidae. One species, Mesocricetus auratus or golden hamster is widely used in biomedical research. Hamsters, Golden,Hamsters, Golden Syrian,Hamsters, Syrian,Mesocricetus auratus,Syrian Golden Hamster,Syrian Hamster,Golden Hamster,Golden Hamster, Syrian,Golden Hamsters,Golden Syrian Hamsters,Hamster, Golden,Hamster, Syrian,Hamster, Syrian Golden,Syrian Hamsters
D012152 Rete Testis The network of channels formed at the termination of the straight SEMINIFEROUS TUBULES in the mediastinum testis. Rete testis channels drain into the efferent ductules that pass into the caput EPIDIDYMIS. Testis, Rete
D004822 Epididymis The convoluted cordlike structure attached to the posterior of the TESTIS. Epididymis consists of the head (caput), the body (corpus), and the tail (cauda). A network of ducts leaving the testis joins into a common epididymal tubule proper which provides the transport, storage, and maturation of SPERMATOZOA.
D005260 Female Females
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D000177 Acrosome The cap-like structure covering the anterior portion of SPERM HEAD. Acrosome, derived from LYSOSOMES, is a membrane-bound organelle that contains the required hydrolytic and proteolytic enzymes necessary for sperm penetration of the egg in FERTILIZATION. Acrosomes
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013084 Sperm-Ovum Interactions Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION. Ovum-Sperm Interactions,Sperm Penetration,Egg-Sperm Interactions,Gamete Interactions,Oocyte-Sperm Interactions,Sperm-Egg Interactions,Sperm-Egg Penetration,Sperm-Oocyte Interactions,Sperm-Oocyte Penetration,Sperm-Ovum Penetration,Sperm-Zona Pellucida Penetration,Egg Sperm Interactions,Egg-Sperm Interaction,Gamete Interaction,Oocyte Sperm Interactions,Oocyte-Sperm Interaction,Ovum Sperm Interactions,Ovum-Sperm Interaction,Sperm Egg Interactions,Sperm Egg Penetration,Sperm Oocyte Interactions,Sperm Oocyte Penetration,Sperm Ovum Interactions,Sperm Ovum Penetration,Sperm Penetrations,Sperm Zona Pellucida Penetration,Sperm-Egg Interaction,Sperm-Egg Penetrations,Sperm-Oocyte Interaction,Sperm-Oocyte Penetrations,Sperm-Ovum Interaction,Sperm-Ovum Penetrations,Sperm-Zona Pellucida Penetrations

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