Biomaterial Database

Intracellular injection of neurobiotin or horseradish peroxidase reveals separate types of preganglionic neurons in the sacral parasympathetic nucleus of the cat. 1993

C W Morgan, and W C De Groat, and L A Felkins, and S J Zhang

Department of Anatomy and Neurobiology, Eastern Virginia Medical School, Norfolk 23501.

Sacral preganglionic neurons are essential to the neural control of the excretory and sex organs. Previously employed multi-cell tracing methods have certain limitations in the precise morphological analysis of the neural pathways that control these organs. These limitations were overcome by the intracellular injection of neurobiotin or horseradish peroxidase into single preganglionic neurons in the lateral sacral parasympathetic nucleus of the cat. Following light microscopic examination, these neurons, as a group, were found to have an average of five stem dendrites, which divided into 15 dendritic end-branches that were distributed among eight dendritic terminal fields. These dendrites had a major transverse orientation and were quite long, many of them reaching well into the dorsal and ventral horns and into the dorsal gray commissure. These dendrites also exhibited a major longitudinal orientation, extending an average of 869 microns (combined length of rostral and caudal dendrites) within the nucleus. Two groups of cells emerged on the basis of different dendritic patterns. Cells classed as Type I had dendrites in lamina I and in the ventral horn but lacked a significant projection into the lateral funiculus. Cells classed as Type II had major dendritic projections into the lateral funiculus but lacked dendrites in lamina I. The diverse dendritic patterns of these two cell types indicate dissimilar afferent control mechanisms and suggest that these preganglionic neurons may innervate different target organs.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008845	Microinjections	The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.	Microinjection
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D003712	Dendrites	Extensions of the nerve cell body. They are short and branched and receive stimuli from other NEURONS.	Dendrite
D005260	Female		Females
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001339	Autonomic Fibers, Preganglionic	NERVE FIBERS which project from the central nervous system to AUTONOMIC GANGLIA. In the sympathetic division most preganglionic fibers originate with neurons in the intermediolateral column of the SPINAL CORD, exit via ventral roots from upper thoracic through lower lumbar segments, and project to the paravertebral ganglia; there they either terminate in SYNAPSES or continue through the SPLANCHNIC NERVES to the prevertebral ganglia. In the parasympathetic division the fibers originate in neurons of the BRAIN STEM and sacral spinal cord. In both divisions the principal transmitter is ACETYLCHOLINE but peptide cotransmitters may also be released.	Autonomic Fiber, Preganglionic,Fiber, Preganglionic Autonomic,Fibers, Preganglionic Autonomic,Preganglionic Autonomic Fiber,Preganglionic Autonomic Fibers
D001369	Axons	Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.	Axon