To obtain insight into the noradrenergic system of amphibians, the distribution of noradrenaline was studied immunohistochemically with antibodies against noradrenaline (NA) and dopamine-beta-hydroxylase (DBH) in the brain of the South African clawed frog Xenopus laevis. Noradrenaline-containing cell bodies are found in the hypothalamic periventricular organ, the isthmic region, and in an area ventral and medial to the solitary tract. Noradrenaline-immunoreactive (NAi) fibers are widely, but not uniformly, distributed throughout the brain and spinal cord. In the telencephalon, dense plexuses of NAi fibers are present dorsomedial to the nucleus accumbens, in the nucleus of the diagonal band, the dorsolateral part of the striatum, the medial amygdala, and in an area that encompasses the lateral forebrain bundle. In the diencephalon, dense plexuses are found ventrolateral to the periventricular organ, in the posterior tubercle, and in the intermediate lobe of the hypophysis. Compared to the forebrain, the brainstem and spinal cord are less densely innervated by NAi fibers. The distribution of DBHi cell bodies and fibers resembles the pattern revealed with the NA antibodies. An exception is formed by the liquor contacting cells of the hypothalamic periventricular organ, which are immunonegative for the DBH antiserum. It is suggested that these cells accumulate rather than metabolize catecholamines. The present study combined with the results of a previous report in Xenopus on the distribution of dopamine (González, Tuinhof, Smeets, '93, Anat. Embryol. 187:193-201) offers the opportunity to differentiate between the two catecholamines. For example, it is now shown that both dopaminergic and noradrenergic fibers innervate the intermediate lobe of the hypophysis and that, therefore, both catecholamines are likely involved in background adaptation.