BIOMAT Database Logo BIOMAT Database Logo

Genetic typing of HLA class II genes in Swedish populations: application to forensic analysis. 1993

M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
Department of Medical Genetics, University of Uppsala Biomedical Center, Sweden.

In an attempt to determine the value of DNA based typing of HLA class II loci to forensic analysis, allele and genotype frequencies at DQA1, DQB1, DPB1, and DRB1 were determined in samples from two Swedish populations using hybridization with sequence specific oligonucleotides to PCR amplified DNA. Significant allele frequency differences were observed at the DQB1 and DRB1 loci between the two populations, as well as between one of the Swedish and a Norwegian population. The average heterozygosity varies between 0.74 to 0.91 and the power of discrimination between 0.90 to 0.98, with the highest values obtained for the DRB1 locus. The probability of genotype identity by chance differs on average 2% between the populations. When applied to a paternity case with one parent deceased and a criminal case, typing of class II loci proved in both cases informative. Analyses of DR and DQ genes does not increase the power of discrimination, due to strong linkage, but offers through the reconstruction of putative haplotypes an internal control for the consistency of the typing results at several loci. Typing of the DRB1 and DPB1 loci was found to result in an approximate combined average probability of genotype identity by chance of one in a thousand.

UI MeSH Term Description Entries
D008297 Male Males
D010334 Paternity Establishing the father relationship of a man and a child. Paternities
D011902 Rape Sexual intercourse without consent of the victim.
D005554 Forensic Medicine The application of medical knowledge to questions of law. Legal Medicine,Medicine, Forensic,Medicine, Legal
D005784 Gene Amplification A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication. Amplification, Gene
D005802 Genes, MHC Class II Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and include H-2M, I-A, and I-E loci in mice. Class II Genes,Genes, Class II,Genes, HLA Class II,MHC Class II Genes,Class II Gene,Gene, Class II
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D006197 Hair A filament-like structure consisting of a shaft which projects to the surface of the SKIN from a root which is softer than the shaft and lodges in the cavity of a HAIR FOLLICLE. It is found on most surfaces of the body. Fetal Hair,Hair, Fetal,Lanugo,Fetal Hairs,Hairs,Hairs, Fetal
D006683 HLA-DQ Antigens A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases. HLA-DC Antigens,HLA-MB Antigens,HLA-DC,HLA-DQ,HLA-DS,HLA-DS Antigens,HLA-LB,HLA-LB Antigens,HLA-MB,Antigens, HLA-DC,Antigens, HLA-DQ,Antigens, HLA-DS,Antigens, HLA-LB,Antigens, HLA-MB,HLA DC Antigens,HLA DQ Antigens,HLA DS Antigens,HLA LB Antigens,HLA MB Antigens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
HLA class II genes: typing by DNA analysis.
April 1990, Bailliere's clinical haematology,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
[DNA typing of HLA class II genes].
December 1990, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
HLA class II DNA typing in two Brazilian populations.
May 1993, Tissue antigens,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
[DNA typing of HLA-class II genes in idiopathic nephropathy].
December 1992, Nihon rinsho. Japanese journal of clinical medicine,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
Molecular HLA-class II typing: advantages and clinical application.
January 1991, Nouvelle revue francaise d'hematologie,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
Molecular analysis of HLA Class I and Class II genes in four indigenous Malaysian populations.
February 2010, Tissue antigens,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
Linkage analysis of HLA class II genes in Swedish multiplex families with multiple sclerosis.
March 1997, Neurology,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
Genomic typing for HLA class II antigens: application to matching of renal allografts.
February 1987, Transplantation proceedings,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
Use of nonisotopic M13 probes for genetic analysis: application to HLA class II loci.
December 1986, Proceedings of the National Academy of Sciences of the United States of America,
M Allen, and T Saldeen, and U Pettersson, and U Gyllensten
PCR-based HLA class II typing.
November 1991, PCR methods and applications,
Copied contents to your clipboard!