BIOMAT Database Logo BIOMAT Database Logo

Molecular karyotype characterization of Leishmania panamensis, Leishmania mexicana, and Leishmania major-like parasites: agents of cutaneous leishmaniasis in Ecuador. 1993

K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Department of Parasitology, Jikei University School of Medicine, Tokyo, Japan.

Molecular karyotypes of Leishmania isolates from patients with cutaneous leishmaniasis in Ecuador were analyzed by pulsed-field gel electrophoresis (PFGE) and Southern blot hybridization. The DNA karyotypes of L. major-like parasites were similar between two human isolates from a lowland coastal and a highland Andean region, but were apparently different from those of eleven World Health Organization reference strains including L. major. The smallest chromosome of 240 kilobases in L. major-like parasites was found to belong to the 715-class of small linear chromosomal DNAs, which have been shown to appear in some lines of Leishmania. Chromosome banding patterns of L. mexicana isolates exhibited a novel, ordered, chromosomal ladder, and were identical among four human isolates and one canine isolate from a restricted geographic region in the Andes. On the other hand, minor chromosome size polymorphisms were observed among three L. panamensis isolates from different endemic regions near the Pacific Coast. Chromosomal locations of dihydrofolate reductase-thymidylate synthetase and P-glycoprotein genes revealed further differences in chromosomal organizations among these Leishmania species in Ecuador. These results indicate that karyotype analysis by PFGE is useful for epidemiologic studies of leishmaniasis in Ecuador.

UI MeSH Term Description Entries
D007621 Karyotyping Mapping of the KARYOTYPE of a cell. Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D007891 Leishmania A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi. Leishmania (Leishmania),Leishmania (Viannia),Leishmania leishmania,Leishmania viannia,Leishmania leishmanias,Leishmania viannias,Leishmanias,Leishmanias (Leishmania),Leishmanias (Viannia),leishmanias, Leishmania,viannias, Leishmania
D007894 Leishmania mexicana A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals including rodents. The Leishmania mexicana complex causes both cutaneous (LEISHMANIASIS, CUTANEOUS) and diffuse cutaneous leishmaniasis (LEISHMANIASIS, DIFFUSE CUTANEOUS) and includes the subspecies amazonensis, garnhami, mexicana, pifanoi, and venezuelensis. L. m. mexicana causes chiclero ulcer, a form of cutaneous leishmaniasis (LEISHMANIASIS, CUTANEOUS) in the New World. The sandfly, Lutzomyia, appears to be the vector. Leishmania (Leishmania) mexicana,Leishmania mexicana amazonensis,Leishmania mexicana mexicana,Leishmania leishmania mexicana,Leishmania leishmania mexicanas,Leishmania mexicana amazonenses,Leishmania mexicana mexicanas,Leishmania mexicanas,amazonenses, Leishmania mexicana,amazonensis, Leishmania mexicana,leishmania mexicana, Leishmania,mexicana amazonensis, Leishmania,mexicana mexicana, Leishmania,mexicana mexicanas, Leishmania,mexicana, Leishmania,mexicana, Leishmania leishmania,mexicana, Leishmania mexicana,mexicanas, Leishmania leishmania
D007895 Leishmania tropica A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects humans and rodents. This taxonomic complex includes species which cause a disease called Oriental sore which is a form of cutaneous leishmaniasis (LEISHMANIASIS, CUTANEOUS) of the Old World. Leishmania (Leishmania) tropica,Leishmania tropica minor,Leishmania leishmania tropica,Leishmania tropicas
D004484 Ecuador A country in western South America, bordering the Pacific Ocean at the equator, between Colombia and Peru. Galapagos Islands
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013762 Tetrahydrofolate Dehydrogenase An enzyme of the oxidoreductase class that catalyzes the reaction 7,8-dihyrofolate and NADPH to yield 5,6,7,8-tetrahydrofolate and NADPH+, producing reduced folate for amino acid metabolism, purine ring synthesis, and the formation of deoxythymidine monophosphate. Methotrexate and other folic acid antagonists used as chemotherapeutic drugs act by inhibiting this enzyme. (Dorland, 27th ed) EC 1.5.1.3. Dihydrofolate Dehydrogenase,Dihydrofolate Reductase,Folic Acid Reductase,Acid Reductase, Folic,Dehydrogenase, Dihydrofolate,Dehydrogenase, Tetrahydrofolate,Reductase, Dihydrofolate,Reductase, Folic Acid
D013940 Thymidylate Synthase An enzyme of the transferase class that catalyzes the reaction 5,10-methylenetetrahydrofolate and dUMP to dihydrofolate and dTMP in the synthesis of thymidine triphosphate. (From Dorland, 27th ed) EC 2.1.1.45. Thymidylate Synthetase,Synthase, Thymidylate,Synthetase, Thymidylate

Related Publications

K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Andean leishmaniasis in Ecuador caused by infection with Leishmania mexicana and L. major-like parasites.
February 1991, The American journal of tropical medicine and hygiene,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Leishmaniasis recidiva cutis due to Leishmania (Viannia) panamensis in subtropical Ecuador: isoenzymatic characterization.
February 2006, International journal of dermatology,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
[Leishmaniasis in Ecuador. 3. Lutzomyia trapidoi, vector of Leishmania panamensis].
March 1994, Annales de la Societe belge de medecine tropicale,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
[Leishmaniasis in Ecuador. 4. Natural infestation of the dog by Leishmania panamensis].
March 1994, Annales de la Societe belge de medecine tropicale,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Genetic analysis of leishmania parasites in Ecuador: are Leishmania (Viannia) panamensis and Leishmania (V.) Guyanensis distinct taxa?
November 1999, The American journal of tropical medicine and hygiene,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Efficacy of ketoconazole against Leishmania braziliensis panamensis cutaneous leishmaniasis.
August 1990, The American journal of medicine,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Tissue eosinophilia and Leishmania mexicana mexicana eosinophil interactions in murine cutaneous leishmaniasis.
September 1984, Parasite immunology,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Draft Genome Sequences of Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) mexicana, and Leishmania (Leishmania) aethiopica, Potential Etiological Agents of Diffuse Cutaneous Leishmaniasis.
May 2019, Microbiology resource announcements,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Disseminated Cutaneous Leishmaniasis in a Patient Infected by Leishmania panamensis.
March 2019, The American journal of tropical medicine and hygiene,
K Katakura, and Y Matsumoto, and E A Gomez, and M Furuya, and Y Hashiguchi
Cutaneous leishmaniasis caused by Leishmania (Viannia) panamensis in 2 travelers.
March 2016, JAAD case reports,
Copied contents to your clipboard!