Biomaterial Database

Thin-layer reversed-phase ion-pair chromatography of some sulphonamides. 1993

M L Bieganowska, and A Petruczynik, and A Doraczynska-Szopa

Department of Inorganic and Analytical Chemistry, Medical Academy, Lublin, Poland.

The retention behaviour of 15 sulphonamides was investigated by thin-layer reversed-phase ion-pair chromatography (RP-IPC). Optimization of the retention and selectivity of these compounds was carried out by changing the pH, the concentration of the ion-pairing counter-ion and the concentration of the organic solvent in the aqueous mobile phase. The effects of various cationic and anionic pairing reagents in the mobile phase and the stationary phase were investigated. The use of stepwise gradient elution improved the spot shape and the selectivity of the separation.

UI	MeSH Term	Description	Entries
D002412	Cations	Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.	Cation
D002855	Chromatography, Thin Layer	Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000838	Anions	Negatively charged atoms, radicals or groups of atoms which travel to the anode or positive pole during electrolysis.	Anion
D012997	Solvents	Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)	Solvent
D013449	Sulfonamides	A group of compounds that contain the structure SO2NH2.	Sulfonamide,Sulfonamide Mixture,Sulfonamide Mixtures,Mixture, Sulfonamide,Mixtures, Sulfonamide