-transparent.png){kind=logo}
Clathrin-coated vesicles transport selected integral membrane proteins from the cell surface and the trans-Golgi network to the endosomal system. Before fusing with their target the vesicles must be stripped of their coats. This process is effected by the chaperone protein hsp70c together with a 100K cofactor which we here identify as the coat protein auxilin. Auxilin binds with high affinity to assembled clathrin lattices and, in the presence of ATP, recruits hsp70c. Dissociation of the lattice does not depend as previously supposed on clathrin light chains or on the amino-terminal domain of the heavy chain. The presence of a J-domain at its carboxy terminus now defines auxilin as a member of the DnaJ protein family. In conjunction with hsp70, DnaJ proteins catalyse protein folding, protein transport across membranes and the selective disruption of protein-protein interactions. We show that deletion of the J-domain of auxilin results in the loss of cofactor activity.
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
October 2001,
Neuron,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
March 2004,
Biochemistry,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
January 2001,
Current biology : CB,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
September 1993,
Plant physiology,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
July 2006,
Proceedings of the National Academy of Sciences of the United States of America,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
April 1990,
The Journal of biological chemistry,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
January 2000,
The Journal of biological chemistry,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
March 1995,
Journal of cell science,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
December 2006,
Neuron,
E Ungewickell, and
H Ungewickell, and
S E Holstein, and
R Lindner, and
K Prasad, and
W Barouch, and
B Martin, and
L E Greene, and
E Eisenberg
March 2010,
Proceedings of the National Academy of Sciences of the United States of America,
