Biomaterial Database

Participation of delta opioid receptor subtypes in the stimulation of adenylyl cyclase activity in rat olfactory bulb. 1995

M C Olianas, and P Onali

Department of Neurosciences, University of Cagliari, Italy.

A number of recent investigations have proposed the existence of two pharmacologically distinct delta opioid receptor subtypes, named delta 1 and delta 2. In the present study, we have investigated the involvement of the two delta receptors in the opioid stimulation of adenylyl cyclase activity in rat olfactory bulb. In addition, we have conducted a similar investigation in rat striatum, where delta agonists are known to inhibit cyclic AMP formation. Both (D-Ala2, Glu4) deltorphin (DELT), a delta 2 agonist, and [D-Pen2, D-Pen5] enkephalin (DPDPE), a delta 1 agonist, stimulated adenylyl cyclase activity in rat olfactory bulb in a concentration-dependent manner, DELT being 25-fold more potent than DPDPE. The selective delta 2 antagonist naltriben counteracted the stimulatory effects of both agonists with a potency about 10-fold higher than that of the selective delta 1 antagonist 7-benzylidenenaltrexone. Moreover, pretreatment of olfactory bulb membranes with the nonequilibrium antagonist naltrindole 5'-isothiocyanate, which irreversibly blocks the delta 2 subtype, reduced the stimulatory effects of both DELT and DPDPE, whereas pretreatment with [D-Ala2, Leu5, Cys6]enkephalin, which binds covalently to delta 1 receptors, failed to affect the response to the agonists. Similar results were obtained in rat striatum. These data indicate that delta opioid receptors coupled to either stimulation or inhibition of adenylyl cyclase in two different brain areas predominantly belong to the delta 2 subtype.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009271	Naltrexone	Derivative of noroxymorphone that is the N-cyclopropylmethyl congener of NALOXONE. It is a narcotic antagonist that is effective orally, longer lasting and more potent than naloxone, and has been proposed for the treatment of heroin addiction. The FDA has approved naltrexone for the treatment of alcohol dependence.	Antaxone,Celupan,EN-1639A,Nalorex,Naltrexone Hydrochloride,Nemexin,ReVia,Trexan,EN 1639A,EN1639A
D009830	Olfactory Bulb	Ovoid body resting on the CRIBRIFORM PLATE of the ethmoid bone where the OLFACTORY NERVE terminates. The olfactory bulb contains several types of nerve cells including the mitral cells, on whose DENDRITES the olfactory nerve synapses, forming the olfactory glomeruli. The accessory olfactory bulb, which receives the projection from the VOMERONASAL ORGAN via the vomeronasal nerve, is also included here.	Accessory Olfactory Bulb,Olfactory Tract,Bulbus Olfactorius,Lateral Olfactory Tract,Main Olfactory Bulb,Olfactory Glomerulus,Accessory Olfactory Bulbs,Bulb, Accessory Olfactory,Bulb, Main Olfactory,Bulb, Olfactory,Bulbs, Accessory Olfactory,Bulbs, Main Olfactory,Bulbs, Olfactory,Glomerulus, Olfactory,Lateral Olfactory Tracts,Main Olfactory Bulbs,Olfactorius, Bulbus,Olfactory Bulb, Accessory,Olfactory Bulb, Main,Olfactory Bulbs,Olfactory Bulbs, Accessory,Olfactory Bulbs, Main,Olfactory Tract, Lateral,Olfactory Tracts,Olfactory Tracts, Lateral,Tract, Lateral Olfactory,Tract, Olfactory,Tracts, Lateral Olfactory,Tracts, Olfactory
D003342	Corpus Striatum	Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.	Lenticular Nucleus,Lentiform Nucleus,Lentiform Nuclei,Nucleus Lentiformis,Lentiformis, Nucleus,Nuclei, Lentiform,Nucleus, Lenticular,Nucleus, Lentiform,Striatum, Corpus
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D000262	Adenylyl Cyclases	Enzymes of the lyase class that catalyze the formation of CYCLIC AMP and pyrophosphate from ATP.	Adenyl Cyclase,Adenylate Cyclase,3',5'-cyclic AMP Synthetase,Adenylyl Cyclase,3',5' cyclic AMP Synthetase,AMP Synthetase, 3',5'-cyclic,Cyclase, Adenyl,Cyclase, Adenylate,Cyclase, Adenylyl,Cyclases, Adenylyl,Synthetase, 3',5'-cyclic AMP
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016308	Enkephalin, Leucine-2-Alanine	A delta-selective opioid (ANALGESICS, OPIOID). It can cause transient depression of mean arterial blood pressure and heart rate.	2-Alanyl-Leucine Enkephalin,5-Leucine-2-Alanine Enkephalin,D-Ala(2)-D-Leu(5)-Enkephalin,Enkephalin-Leu,Ala(2),Leucine Enkephalin-2-Alanine,BW-180C,DADLE,Leu-Enkephalin-2-Ala,2 Alanyl Leucine Enkephalin,5 Leucine 2 Alanine Enkephalin,BW 180C,BW180C,Enkephalin, 2-Alanyl-Leucine,Enkephalin, 5-Leucine-2-Alanine,Enkephalin, Leucine 2 Alanine,Enkephalin-2-Alanine, Leucine,Leu Enkephalin 2 Ala,Leucine Enkephalin 2 Alanine,Leucine-2-Alanine Enkephalin