Biomaterial Database

Immunosuppressive activity of a porcine high-molecular weight uterine macromolecule is associated with transforming growth factor-beta. 1995

E C Segerson

Department of Animal Science, North Carolina Agricultural and Technical State University, Greensboro 27411, USA.

A > or = 230 kDa lymphocyte suppressor component recovered from uterine luminal protein (ULP) secretions of Landrace and Yorkshire gilts on day 15 of pregnancy was further purified by anion-exchange (DEAE Sepharose CL-6B) and gel filtration (Sepharose CL-6B) chromatography. Macromolecules within each peak fraction were tested for suppression of thymidine (TdR) incorporation into phytohemagglutinin (PHA)-treated peripheral blood lymphocytes (PBL). A neutralizing antibody to transforming growth factor-beta (TGF-beta) was added to additional cultures in order to determine whether suppressor activity of high-molecular weight macromolecules was associated with TGF-beta. The > or = 230 kDa component suppressed (P < 0.0005) TdR incorporation into PHA-treated PBL and anti-TGF-beta reversed (P < 0.034) the suppressor response. Following anion-exchange chromatography of this component, suppressor activity was greatest (P < 0.05) for an acidic component (fraction III), which comprised 45.9% of eluted macromolecules. Sepharose CL-6B chromatography of fraction III resulted in a major (58.7% of eluted macromolecules) component (> or = 4 x 10(6) Da (> or = 4 MDa), eluted at the void volume) which suppressed (P < 0.05) TdR incorporation into PHA-treated PBL. Anti-TGF-beta reduced the suppressor activity of this macromolecule by 34.2%. Suppressor responses were not associated with viabilities of PBL. These data demonstrate that ULP secretions from gilts on day 15 of pregnancy contain a > or = 4 MDa macromolecule which suppresses proliferative responses of PBL and likely serves as a carrier for TGF-beta.

UI	MeSH Term	Description	Entries
D007108	Immune Tolerance	The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.	Immunosuppression (Physiology),Immunosuppressions (Physiology),Tolerance, Immune
D007166	Immunosuppressive Agents	Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.	Immunosuppressant,Immunosuppressive Agent,Immunosuppressants,Agent, Immunosuppressive,Agents, Immunosuppressive
D008213	Lymphocyte Activation	Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.	Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011270	Pregnancy, Animal	The process of bearing developing young (EMBRYOS or FETUSES) in utero in non-human mammals, beginning from FERTILIZATION to BIRTH.	Animal Pregnancies,Animal Pregnancy,Pregnancies, Animal
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D002852	Chromatography, Ion Exchange	Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.	Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D005260	Female		Females
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated