Micro-imaging displays of the anterior segment of the eye, such as of the anterior chamber angle, iris, ciliary body, ciliary zonule of Zinn, and anterior surface of the crystalline lens, were obtained in human eyes in vivo by means of a recently developed, high-frequency, high-resolution ultrasonic diagnostic unit (30 MHz). Much clearer displays than conventional ultrasonic imaging displays (5-15 MHz) were obtained, showing improved resolution, and greater morphologic diagnostic information was provided. Displays considered useful for making measurements were also provided. The subjects were 20 normal volunteers (35 eyes), 36 patients with glaucoma (58 eyes), 6 patients with uveal diseases (6 eyes), and 28 patients with pseudophakia (32 eyes). The equipment used was a model UX-02 ultrasonic diagnostic unit (RION), and the transducer was the three-element annular array type. The frequency was 30 MHz, the resolution was below 50 microns, and tissue penetration was 6 mm. This method enabled close ultrasound biomicroscopic imaging observations of details of the anterior and posterior chambers of the eyes in vivo and was also useful for clinical diagnosis and elucidation of the cause of glaucoma of various types. It also for the first time enabled evaluation of the position of fixation of an intraocular lens loop in the pseudophakic eyes containing an intraocular lens.