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We describe the expression of gpIRK1, an inwardly rectifying K+ channel obtained from guinea pig cardiac cDNA. gpIRK1 is a homologue of the mouse IRK1 channel identified in macrophage cells. Expression of gpIRK1 in Xenopus oocytes produces inwardly rectifying K+ current, similar to the cardiac inward rectifier current IK1. This current is blocked by external Ba2+ and Cs+. Plasmids containing the gpIRK1 coding region under the transcriptional control of constitutive (PGK) or inducible (GAL) promoters were constructed for expression in Saccharomyces cerevisiae. Several observations suggest that gpIRK1 forms functional ion channels when expressed in yeast. gpIRK1 complements a trk1 delta trk2 delta strain, which is defective in potassium uptake. Expression of gpIRK1 in this mutant restores growth on low potassium media. Growth dependent on gpIRK1 is inhibited by external Cs+. The strain expressing gpIRK1 provides a versatile genetic system for studying the assembly and composition of inwardly rectifying K+ channels.
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
March 1995,
Circulation research,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
September 2000,
American journal of physiology. Cell physiology,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
January 1995,
Receptors & channels,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
January 1991,
Annual review of physiology,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
July 1994,
Brain research. Molecular brain research,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
November 1997,
The Journal of physiology,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
March 2011,
Pflugers Archiv : European journal of physiology,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
October 1997,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
December 1996,
FEBS letters,
W Tang, and
A Ruknudin, and
W P Yang, and
S Y Shaw, and
A Knickerbocker, and
S Kurtz
October 2012,
Development (Cambridge, England),
