-transparent.png){kind=logo}
We investigated the secretion of TNF-alpha by monocytes and macrophages derived from the peripheral blood, spleen, and lungs after a single exposure to a therapeutic profile of hyperbaric oxygen (HBO). Rats were exposed for 90 min to either 100% oxygen at 0.28 MPa (2.8 atmospheres absolute) or air. Immediately after exposure, mononuclear cells were isolated from blood, spleen, and lungs and cultured for 18 h. The secretion of TNF-alpha from the cultured monocytes/macrophages was determined with and without stimulation with lipopolysaccharide (LPS). Exposure to hyperbaric oxygen induced a significant increase in the spontaneous ex vivo secretion of TNF-alpha (without LPS) by mononuclear cells from the blood, spleen, and lung (P < 0.05 from air controls). Stimulation with LPS after exposure to HBO induced a significant increase in TNF-alpha secretion by lung and spleen macrophages compared with air controls (P < 0.05). However, absolute TNF-alpha levels were not significantly higher than those achieved 'spontaneously' in macrophages exposed to HBO without LPS. Stimulation with LPS induced a marked increase in secretion of TNF-alpha from blood monocytes after exposure to air, but not after exposure to HBO. These results provide evidence in support of a role played by TNF-alpha in mediating HBO effects on different tissues and their immune responses.
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
August 1998,
Clinical and laboratory haematology,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
January 1993,
Immunology,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
November 1987,
Blood,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
June 2003,
Cell biochemistry and function,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
August 2000,
Clinical and experimental immunology,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
April 1996,
Journal of the Egyptian Society of Parasitology,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
May 2001,
Diabetologia,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
February 2002,
Phytotherapy research : PTR,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
January 2001,
Clinical and experimental immunology,
N Lahat, and
H Bitterman, and
N Yaniv, and
A Kinarty, and
N Bitterman
January 1995,
Reproduction, fertility, and development,
