The distribution of FMRFamide-like immunoreactive peptides was studied in the brain of the African lungfish, Protopterus annectens, using the indirect immunofluorescence technique. The main populations of FMRFamide-positive cell bodies were detected in the forebrain and in the mesencephalic tegmentum. In the telencephalon, only a small number of FMRFamide-immunoreactive neurons was localized at the level of the subpallium, in the nucleus septi medialis. The diencephalon contained two prominent groups of FMRFamide-positive cell bodies located in the preoptic and periventricular preoptic nuclei. The thalamus exhibited only scattered FMRFamide-immunoreactive perikarya in its ventral part. In the mesencephalon, a group of positive cell bodies was identified in the caudal region of the tegmentum. A strong immunoreaction was also detected in the nervus terminalis. In the pituitary, most of the cells of the intermediate lobe were brightly stained. FMRFamide-like immunoreactive fibers and nerve terminals were widely distributed in the brain. In the telecephalon, numerous fibers were observed in several regions of the pallium and subpallium. A dense plexus of fibers was found in the hypothalamus and the thalamus. Immunoreactive fibers were seen coursing along the ventral wall of the infundibular cavity and terminating in the pars nervosa of the pituitary. The tectum and the ventral mesencephalon were also densely innervated. In contrast, the caudal brainstem only showed scarce immunoreactive processes. Coexistence of FMRFamide- and neuropeptide Y-like immunoreactivity was observed in the preoptic nucleus and in the nervus terminalis. The widespread distribution of FMRFamide-immunoreactive neurons in the brain and pituitary of P. annectens suggests that the peptide may exert both neuromodulator and neuroendocrine functions. The similarity between the distribution patterns of FMRFamide and neuropeptide Y in the brain of lungfish and amphibians supports the concept of a close phylogenetic link between these two groups.