-transparent.png){kind=logo}
BACKGROUND Tumor cell-induced platelet aggregation (TCIPA) is considered to be a critical step in hematogenous metastasis. METHODS TCIPA was studied in vitro in six human pancreatic carcinoma cell lines (PC 3, PC 44, AsPC1, BxPC3, Capan2, Panc1). RESULTS Whereas all cell lines induced aggregation of washed platelets in the presence of minimal amounts of platelet-poor plasma, five cell lines also induced aggregation of platelets in platelet-rich plasma. The thrombin-antagonist hirudin inhibited TCIPA in all cell lines indicating that TCIPA is thrombin-dependent. Since pretreatment of tumor cells with phospholipase A2 or C inhibited TCIPA, the thrombin-generating activity might be confined to the tumor cell surface. Further support for a prothrombinase activity was provided by the observation that all cell lines were able to induce the aggregation of washed platelets after addition of purified coagulation factors II and V. CONCLUSIONS Pancreatic carcinoma cells are able to induce platelet aggregation via activation of thrombin. This might support metastasis in pancreatic cancer and possibly explain the incidence of thrombosis in this tumor.
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
January 1996,
Journal of cancer research and clinical oncology,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
January 1982,
Progress in clinical and biological research,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
March 1984,
Gan to kagaku ryoho. Cancer & chemotherapy,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
December 1996,
International journal of pancreatology : official journal of the International Association of Pancreatology,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
June 1987,
Thrombosis research,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
September 1981,
Cancer research,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
January 1969,
Pflugers Archiv : European journal of physiology,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
January 1986,
Invasion & metastasis,
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
December 1987,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
E Heinmöller, and
T Schropp, and
O Kisker, and
B Simon, and
R Seitz, and
R J Weinel
October 1977,
Thrombosis and haemostasis,
