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The majority of human peripheral gamma delta T cells express the V gamma 9 gene in combination with the V delta 2 gene. The diversity of this subset of gamma delta T cells is limited by a preferential usage of the J gamma P gene segment and a highly distinctive junctional motif of the T-cell receptor (TCR) delta chain. We and others have observed that peripheral blood derived V gamma 9+V delta 2+ gamma delta T cells of healthy individuals are activated after stimulation with interleukin-2 (IL-2) in vitro, but only a small percentage of gamma delta T cells subsequently proliferates. To assess whether the proliferating, IL-2-responsive gamma delta T cells represent a selective group of T cells, we have analysed TCR junctional features of IL-2-responsive gamma delta T cells. Out of 30 individuals studied, nine were identified as IL-2-responders and three as IL-2-hyperresponders. The TCR V(D)J gene usage from IL-2 stimulated peripheral blood lymphocytes of these IL-2-(hyper)responsive individuals was analysed. The results showed that in most individuals gamma delta T cells polyclonally expanded after stimulation with IL-2. In two IL-2-hyperresponder individuals, however, a monoclonal expansion of a particular V gamma 9+V delta 2+ gamma delta T cell was found. In one of these individuals, this V gamma 9+V delta 2+ T-cell clone expressed a very rare gamma delta TCR type because of the presence of an Ala within the junctional region at a conserved position relative to V delta framework residues (delta 97), which is very infrequently used by peripheral blood V gamma 9+V delta 2+ cells. This particular clonotype could also be detected in unstimulated PBL samples taken from that individual, and made up for 30% of the total peripheral gamma delta T-cell pool. These data indicate that in general IL-2-responsive V gamma 9+V delta 2+ gamma delta T cells represent a polyclonal population, reflecting in vivo stimulation with multiple antigens or superantigens. In contrast, monoclonal expansions of gamma delta T cells after stimulation with IL-2 can also occur, which may be related to an in vivo stimulation by one particular antigen, rendering this gamma delta T-cell type dominant in the peripheral blood.
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
October 1993,
Immunology letters,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
January 1999,
Springer seminars in immunopathology,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
March 1991,
Seminars in immunology,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
January 1990,
Biomedica biochimica acta,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
April 2003,
European journal of haematology,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
January 1991,
Microbiology and immunology,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
July 1998,
Medicina clinica,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
January 1998,
Ryoikibetsu shokogun shirizu,
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
September 1993,
Journal of immunology (Baltimore, Md. : 1950),
D L Orsini, and
Y M Kooy, and
M A Van der Tol, and
L Struyk, and
P Van den Elsen, and
F Koning
January 1994,
The Journal of experimental medicine,
