[Evaluation of selective culture media for the isolation of Salmonella from poultry]. 1995

M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Argentina.

Rambach agar, xylose-lysine-deoxycholate agar (XLD) with different concentrations of Tergitol 4 or 7 ethyl-2 methyl-4 undecanol hydrogen sulphate, sodium salt (XLDT4), Salmonella-Shigella agar (SS) and bismuth sulfite agar according to Wilson-Blair (BS) were evaluated using Salmonella spp. serovars and other bacterial species from the intestinal flora of poultry. Growth of the most common Salmonella serovars isolated from chickens in our country were evaluated using a viable counting technique on the different selective media and these results were compared with those obtained on Columbia base (ABC) agar plus 7% bovine blood (Table 1). Samples from Salmonella experimentally inoculated chickens were also examined. Results showed that Rambach, SS and XLD or XLDT4 were all satisfactory for isolation of Salmonella. Bismuth Sulfite agar was too inhibitory for bacteria important in veterinary practice. The characteristic colonies of Salmonella and other common fecal contaminant bacteria growing on SS, Rambach, XLDT4 and SB are shown in Table 2. Addition of tergitol or novobiocin to XLD agar did not completely inhibit the growth of all Proteus spp. strains examined. None of the Proteus spp. strains able to multiply on SS, XLD or XLDT4 agar grew on the commercial Rambach agar. Several different contaminant bacterial species produced Salmonella-like colonies on Rambach, SS, XLD and XLDT4 agars. Because these contaminant bacterial species are different it is advisable to improve the diagnosis by culturing samples on SS, XLD or XLDT4 agar and also simultaneously on Rambach agar.

UI MeSH Term Description Entries
D008239 Lysine An essential amino acid. It is often added to animal feed. Enisyl,L-Lysine,Lysine Acetate,Lysine Hydrochloride,Acetate, Lysine,L Lysine
D008460 Meat The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game. Meats
D011060 Poloxalene A copolymer of polyethylene and polypropylene ether glycol. It is a non-ionic polyol surface-active agent used medically as a fecal softener and in cattle for prevention of bloat. Oxyethylene Oxypropylene Polymer,Polyoxyethylene Polyoxypropylene Polyoxyethylene Polymer,Bloat Guard,Poloxalene 2930,Proxanol,SK&F-18667,Tergitol,Therabloat,Oxypropylene Polymer, Oxyethylene,Polymer, Oxyethylene Oxypropylene,SK&F 18667,SK&F18667
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D001729 Bismuth A metallic element that has the atomic symbol Bi, and atomic number 83. Its principal isotope is Bismuth 209.
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D003840 Deoxycholic Acid A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine. Deoxycholic acid acts as a detergent to solubilize fats for intestinal absorption, is reabsorbed itself, and is used as a choleretic and detergent. Deoxycholate,Desoxycholic Acid,Kybella,Choleic Acid,Deoxycholic Acid, 12beta-Isomer,Deoxycholic Acid, 3beta-Isomer,Deoxycholic Acid, 5alpha-Isomer,Deoxycholic Acid, Disodium Salt,Deoxycholic Acid, Magnesium (2:1) Salt,Deoxycholic Acid, Monoammonium Salt,Deoxycholic Acid, Monopotassium Salt,Deoxycholic Acid, Monosodium Salt,Deoxycholic Acid, Sodium Salt, 12beta-Isomer,Dihydroxycholanoic Acid,Lagodeoxycholic Acid,Sodium Deoxycholate,12beta-Isomer Deoxycholic Acid,3beta-Isomer Deoxycholic Acid,5alpha-Isomer Deoxycholic Acid,Deoxycholate, Sodium,Deoxycholic Acid, 12beta Isomer,Deoxycholic Acid, 3beta Isomer,Deoxycholic Acid, 5alpha Isomer
D004755 Enterobacteriaceae A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock. Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis

Related Publications

M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
January 2010, Journal of AOAC International,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
January 1980, Medycyna doswiadczalna i mikrobiologia,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
August 1976, Bollettino della Societa italiana di biologia sperimentale,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
December 1998, FEMS microbiology letters,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
October 1969, Applied microbiology,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
April 1984, Journal of food protection,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
June 1952, Gigiena i sanitariia,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
February 2001, Letters in applied microbiology,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
December 1996, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
M M Yuño, and H R Terzolo, and H D Fernández, and R C Malena, and M E Altuna
March 1952, The Journal of hygiene,
Copied contents to your clipboard!