-transparent.png){kind=logo}
Human interferon-gamma (IFN-gamma) cDNA was synthesized, and it makes the usage of favorable codons in E. coli. The authors got 9 different expression plasmids which contain the synthetic IFN-gamma-cDNA and have different spaces between SD sequence and ATG. The free energies G0f298 in the formation of stable secondary structure in the translation initiation region (TIR) are different in various expression plasmids. One of them, pLY4-gamma 5, can highly yield INF-gamma which will be about 60%-80% of the total bacterial proteins, such a high expression was hardly noted in literature. The reasons of high expression in this work are optimal spaces between SD and ATC, favorable delta G0f298, favourable condons usage for E. coli.
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
March 1992,
Science in China. Series B, Chemistry, life sciences & earth sciences,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
February 1982,
Nature,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
March 1983,
Nucleic acids research,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
December 2009,
Journal of industrial microbiology & biotechnology,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
October 1995,
Science in China. Series B, Chemistry, life sciences & earth sciences,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
May 1983,
Nucleic acids research,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
January 1987,
FEBS letters,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
December 1986,
Biochemical and biophysical research communications,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
August 1992,
Applied biochemistry and biotechnology,
Z Wang, and
D Yang, and
Q Wang, and
B Li, and
Z Lü, and
J Yu, and
H Zheng, and
P Fan, and
J Tang, and
M Qian
February 1988,
Biochemical and biophysical research communications,
