Biomaterial Database

Studies on multiple Pseudomonas aeruginosa isolates from individual burn patients by RFLP, O antigen serotyping and antibiogram analysis. 1995

I A Holder, and K Volpel, and G Ronald, and W Paranchych

Department of Surgery, University of Cincinnati College of Medicine, Ohio, USA.

Multiple isolates of Pseud. aeruginosa from individual burn patients were tested for antibiotic susceptibility-resistance patterns (antibiogram), O serotype lipopolysaccharide and chromosomal DNA restriction fragment length polymorphisms (RFLP) using a PAK pilin gene probe. Some patients were colonized by isolates identical by all three analytical procedures whereas other patients were found where multiple isolates were identical on the basis of serotype and antibiogram analysis, but different on the basis of RFLP analysis. Examples were found where multiple isolates from an individual patient appeared to be identical on the basis of serotyping and RFLP data, but different on the basis of antibiogram. Strains refractory to O serotyping could be characterized by RFLP type. These results indicate that RFLP analysis provides a valuable addition to routine serotyping and antibiogram studies on Pseud. aeruginosa isolates and that significant numbers of burn patients become co-colonized/co-infected with phenotypically diverse strains of this organism.

UI	MeSH Term	Description	Entries
D011550	Pseudomonas aeruginosa	A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.	Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D012150	Polymorphism, Restriction Fragment Length	Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.	RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D002056	Burns	Injuries to tissues caused by contact with heat, steam, chemicals (BURNS, CHEMICAL), electricity (BURNS, ELECTRIC), or the like.	Burn
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D019081	O Antigens	The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	O-Antigen,O-Specific Polysaccharides,O Antigen,O Antigen, Bacterial,O-Antigens,O-Specific Polysaccharide,Antigen, Bacterial O,Antigen, O,Antigens, O,Bacterial O Antigen,O Specific Polysaccharide,O Specific Polysaccharides,Polysaccharide, O-Specific,Polysaccharides, O-Specific