Construction of a YAC contig and an STS map spanning 3.6 megabase pairs in Xp22.1. 1996

D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
MRC Molecular Endocrinology Group, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.

We have constructed a 3.6 Mb sequence tagged sites (STS)-based yeast artificial chromosome (YAC) contig, consisting of 58 individual YAC clones, spanning the region PDHA1 and DXS451 on Xp22.1. In addition to establishing the order of PDHA1, ISPK-1, DXS2504, DXS1528 and the 13 known polymorphic loci as Xpter-PDHA1-DXS443-DXS3424-ISPK-1-DXS12 29-DXS2504-DXS1528-DXS365-DXS7101- DXS1683-DXS1052-DXS274-DXS92-DXS1226-DX S41-DXS989-DXS451-Xcen, we have also developed 35 novel STSs from YAC end clones. These results provide a high density of STS markers (approximately 1 per 70 kb). Furthermore, a detailed long-range restriction map of the contig has been constructed with rare-cutter enzymes and this has refined and verified the physical distances between markers inferred from YAC sizes and their STS content. The integration of the physical mapping data with previous genetic mapping data and the use of STSs and non-chimeric YAC clones reported here should facilitate the construction of a transcript map of this region and the positional cloning of disease genes in this portion of Xp22.1.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D005819 Genetic Markers A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event. Chromosome Markers,DNA Markers,Markers, DNA,Markers, Genetic,Genetic Marker,Marker, Genetic,Chromosome Marker,DNA Marker,Marker, Chromosome,Marker, DNA,Markers, Chromosome
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001482 Base Composition The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid. Base Ratio,G+C Composition,Guanine + Cytosine Composition,G+C Content,GC Composition,GC Content,Guanine + Cytosine Content,Base Compositions,Base Ratios,Composition, Base,Composition, G+C,Composition, GC,Compositions, Base,Compositions, G+C,Compositions, GC,Content, G+C,Content, GC,Contents, G+C,Contents, GC,G+C Compositions,G+C Contents,GC Compositions,GC Contents,Ratio, Base,Ratios, Base
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014960 X Chromosome The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species. Chromosome, X,Chromosomes, X,X Chromosomes
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016324 Sequence Tagged Sites Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database. Sequence-Tagged Sites,Sequence Tagged Site,Sequence-Tagged Site,Site, Sequence Tagged,Site, Sequence-Tagged,Sites, Sequence Tagged,Sites, Sequence-Tagged,Tagged Site, Sequence,Tagged Sites, Sequence

Related Publications

D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
January 1994, Human molecular genetics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
January 1997, Immunogenetics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
March 1995, Genomics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
December 1996, Genomics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
April 1996, Genomics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
September 1996, Genomics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
February 1995, Genomics,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
February 1997, Genome,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
July 1997, Genome research,
D Trump, and G Pilia, and P H Dixon, and C Wooding, and R Thakrar, and S E Leigh, and R Nagaraja, and M P Whyte, and D Schlessinger, and R V Thakker
February 1993, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Copied contents to your clipboard!